10 / 20
Page 33
Notes:
&
24
th
Biotechnology Congress: Research & Innovations
CRISPR Cas9 Technology and Genetic Engineering
Annual Congress on
October 24-25, 2018 | Boston, USA
Journal of Biotechnology & Biomaterials | ISSN: 2155-952X | Volume: 8
Enhanced bioH2 and poly-hydroxyalkanoates production by a co-culture of
Syntrophomonas wolfei
and a photoheterotrophic mixed consortium using a dark-fermentation effluent as substrate
Z Vanegas-Zuniga, O Cortes De Luna, C Nino Navarro
and
I Garcia Pena I Chairez
National Polytechnic Institute, Mexico
N
owadays, the pollution from oil-based derivatives such as gasoline, polyethylene, etc. is getting problematic. One
possible way to overcome this issue is by developing alternative green technologies. The bio-plastics production seems
to be a promising method to reduce the plastics production. Polyhydroxyalkanoates as the copolymers of PHB and PHV
have similar characteristics of the polyethylenes, therefore many applications
Synthrophomonas wolfei (S. wolfei)
and some
photoheterotrophic bacteria are able to produce this polymer from the effluents of organic residues treatment. Moreover, these
microorganisms may produce bioH2 depending on the culture conditions. However, the production of biopolymers based on
the bacteria metabolism is nowadays still more expensive than synthetic production. This condition motivates the research to
optimize the biological process to make it competitive compared to the regular oil-based method. The purpose of this study
was to develop the syntrophic consortium composed by
S. wolfei
and a photoheterotrophic mixed consortium named C4.
This strategy would allow improving the simultaneous production of bioH2 and PHA. The dark-fermentation effluent was
used as a substrate during the photoheterotrophic process. This effluent consists of a complex mixture of volatile fatty acids
including acetic, butyric, lactic, propionic and some others. The data demonstrated the syntrophic activity between
S. wolfei
and C4 based on the comparison of PHA and H
2
productions from the individual and co-culture fermentations. The individual
cultures showed that consortium C4 and
S. wolfei
can use the effluent as a carbon-nitrogen source.
S. wolfei
produced a higher
concentration of bioH2 but lower PHA production compared with C4.The co-culture produced this bioproduct simultaneously,
with 25%PHA and 90mmolv/vH2 at 100 and 75 hours respectively.The profile of volatile fatty acids consumption explained the
interaction between C4 and
S. wolfei
suggesting a mutualism.
Biography
Zaira Jovana Vanegas Zuniga is a Biotechnology Engineer recently graduated from UPIBI at the National Polytechnic Institute. She is working on a research
project named “Enhanced the bio-hydrogen and poly-hydroxyalkanoates production by a co-culture of
Syntrophomonas wolfei
and the photoheterotrophic mixed
consortium using the dark-fermentation effluent as substrate”. Her current research interests are the production of biofuels (Hydrogen and biopolymers) using
residues from agro-industries as carbon-nitrogen source.
ing.zairavanegas@hotmail.comZ Vanegas-Zuniga et al., J Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C4-097