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24
th
Biotechnology Congress: Research & Innovations
CRISPR Cas9 Technology and Genetic Engineering
Annual Congress on
October 24-25, 2018 | Boston, USA
Journal of Biotechnology & Biomaterials | ISSN: 2155-952X | Volume: 8
Genome editing in wheat, polyploid and complex genome
Hikmet Budak
Montana State University, USA
G
enome editing technology is relatively easy to use and more cost-effective than others. By combining versatile tools to
study and modify plants at different molecular levels, the CRISPR/Cas9 system is paving the way toward a new horizon
for basic research and crop development. Wheat (
Triticum aestivum L.
) is a stable crop providing more than 20% of daily
calories intake for humans and has a complex genome formed with the combination of three different genomes: A, B, and D.
The hexaploid wheat genome makes this plant an important model for studying and optimizing the genome editing system.
In our study, we applied the CRISPR/Cas9 genome editing system for abiotic stress response genes and small RNA genes. The
CRISPR/Cas9 genome editing system in wheat was effectively established using different proteins as well.
Biography
Hikmet Budak currently works at the Department of Plant Sciences and Plant Pathology, Montana State University. Hikmet does research in Molecular Biology,
Genetics and Agricultural Plant Science.
hikmet.budak@montana.eduHikmet Budak, J Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C4-097