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.com
Volume 7, Issue 4 (Suppl)
J Biotechnol Biomater, an open access journal
ISSN: 2155-952X
Bio America 2017
October 19-20, 2017
October 19-20, 2017 | New York, USA
18
th
Biotechnology Congress
Influence of growth time and concentration of silver nitrate over the biosynthesis of silver nanoparticles
using white rot fungi
Bjerkandera sp
. Anamorph R1
Jeronimo Osorio
1
, Juliana Osorio
1
, Claudia P Ossa
2
and
Natalia A Gomez
1
1
Universidad de Antioquia, Colombia
2
Universidad de Sao Pablo, Brazil
Statement of the Problem
: Currently, silver nanoparticles (AgNPs) are being used in areas such as medicine, catalysis, optics and
bactericidal sensors; for this reason, the development of recent methodologies allows a more efficient production of AgNPs with better
antimicrobial and antitoxic properties. The obtention of AgNPs by physical methods tend to produce low amounts of nanoparticles,
while chemical methods are often dangerous and require the use of stabilizing agents. Biological synthesis from white rot fungi are
an alternative to improve these processes and reduce the generation of harmful toxic wastes. This work studies how
Bjerkandera sp
.
Anamorph R1 is affected both by growth time as well concentration of silver nitrate (AgNO
3
) over AgNPs synthesis.
Methodology & Theoretical Orientation:
The synthesis of AgNPs was carried out in two ways: bio-reduction of silver ions by
proteins secreted in the culture broth, as well as, by absorption of silver atom on the mycelia-pellet; to do so, the fungus was grown
for 3-8 days, then the mycelium was separated from the culture broth. Both fractions were mixed with AgNO3 (0.5, 1 and 1.5 mM)
evaluated at different time (24h, 48, 72h, 96h, 120h and 144h).
Findings:
The action of the capping proteins on the surface of the mycelium played a determining role in the reduction of the Ag+
ion to Ag0 nanoparticles producing a particle size that oscillated between 10-100 nm.
Conclusion & Significance:
The operational conditions at which the incubated fungi were maintained improved both the adsorption
of the silver ions on the surface of the mycelium and subsequent synthesis of AgNPs. The best synthetic properties were found at 1mM
of AgNO
3
concentration, growth time of 8 days, and incubation time of 144 hours
jeronimo.osorio@udea.edu.coJ Biotechnol Biomater 2017, 7:4 (Suppl)
DOI: 10.4172/2155-952X-C1-080