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.com
Volume 7, Issue 4 (Suppl)
J Biotechnol Biomater, an open access journal
ISSN: 2155-952X
Bio America 2017
October 19-20, 2017
October 19-20, 2017 | New York, USA
18
th
Biotechnology Congress
Plant cell and tissue cultures as experimental models for studies of coumarin and chlorogenic acid
biosynthesis in
Mikania laevigata
(Asteraceae) and UHPLC-MS analysis
Aranha Netto L, Forato J, Andreazza N L, Sawaya A C H F
and
Salvador M J
State University of Campinas, Brazil
Statement of the Problem
:
Mikania laevigata
, guaco, is commonly used as treatment for respiratory system diseases due to its
bronchodilator effect, bioactivity assigned to coumarin. Plant cell cultures are very promising tolls to produce secondary metabolites
in vitro
. They allow a rapid cell proliferation, they are under strict controlled conditions and are easy to manipulate. This research
aimed to investigate the use of tissue and cell cultures of
M. laevigata
as experimental models applied to the study of coumarin and
chlorogenic acids biosynthesis in this plant.
Methodology & Theoretical Orientation
: Explants obtained from young leaves were sterilized and maintained on MS
medium supplemented with 30 g.L-1 of sucrose and sub-cultured every 30 days. After several tests with different proportions
of hormones to determine their optimal concentrations, medium containing 4.52 µM of 2,4-dichlorophenoxyacetic acid and
26 µM 6-benzilaminopurina was established as the best condition for callus induction for this species. Following the successful
establishment of the callus culture, cell suspensions were established by inoculating cells from friable calli into liquid MS medium.
Analysis for identification and quantification of the phenylpropanoid biosynthetic pathway products were done by UHPLC-MS,
using methodology described by Melo.
Findings:
The analysis of the chemical profile of
M. laevigata
calli, after 15, 30 and 60 days of subculture, detected mainly coumarin,
caffeoylquinic acid and dicaffeoylquinic acid. The highest concentration of coumarin was observed after 30 days, 0.12 ± 0.01 mg.g-1
dry mass. The highest quantification of caffeoylquinic acid was observed after 15 days, which contained 5.53 ± 1.29 mg.g-1 of dry
mass, while dicaffeoylquinic acid had a higher concentration after 60 days, with 36.39 ± 4.58 mg.g-1 dry mass.
Conclusion:
Tissue and cell cultures of
M. laevigata
are promising biological models for application in studies of biosynthesis of its
bioactive secondary metabolites. However, to confirm their full potential, the continuity of the investigations is necessary
Biography
Aranha Netto L is currently a Master's student in the Plant Biology Program at the Institute of Biology - Unicamp. Her research investigates the use of plant cell and tissue
cultures as biological models applied to the study of coumarin and chlorogenic acid biosynthesis in
Mikania glomerata
and
Mikania laevigate
(Asteraceae). She holds a
Bachelor's degree in Biological Sciences from the State University of Campinas (2015), including a year studying at the University of East Anglia (UEA), England as part of
the exchange program Science without borders. Also in England, she did a research placement at Inspiralis Ltd
l117630@dac.unicamp.brAranha Netto L et al., J Biotechnol Biomater 2017, 7:4 (Suppl)
DOI: 10.4172/2155-952X-C1-080