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Volume 7 Journal of Infectious Diseases & Therapy
Page 46
Bacterial and Rare Diseases 2019
June 17-18, 2019
June 17-18, 2019 Dubai, UAE
Joint Meeting on
&
2nd Annual Congress on Bacterial, Viral and Infectious Diseases
6th International Conference on Rare Diseases & Orphan Drug
C Anitha et al., J Infect Dis Ther 2019, Volume 7
Screening of invitro activity of antimicrobial and antibiofilmproperty of Mesenchymal
stems cells againstMDRgramnegative organism isolated fromurinary tract infections
in tertiary care hospital
C Anitha, Sivasankari S, Senthamarai S, Muthulakshmi K, Somasunder V M, Akila K, Sijimol and Subha V J
Meenakshi Medical College Hospital and Research Institute, India
Introduction & Objective: Recent studies had showed that Mesenchymal Stem Cells (MSCs) have beneficial
effects on bacterial infections. Treatment withMSCs has proven bacterial clearance.This study was undertaken
to study the in vitro activity of antimicrobial and antibiofilm activity of stems cells against gram negative
multidrug resistant organism from urinary tract infections.
Method: The samples will be processed according to standard protocol following standard guidelines.
All the isolates obtained will be identified by standard guidelines. Total of 50 isolates were collected. The
antibiotic susceptibility testing will be done for all the isolates by Kirby Bauer disc diffusion method following
CLSI guidelines. All the isolates are screened for production of biofilm by tissue culture plate method. The
antimicrobial activity of mesenchymal stem cells was done by micro broth dilution method.
Results: Among the 50 gram negative isolates 22 (44%) were Pseudomonas species 12 (24%) were E coli 8
(16%) were Klebsiella spp and 8 (16%) were Proteus species. Among the 50 isolates 32 (64%) were multi drug
resistant to the antibiotics tested. Among the 50 isolates 43 (86%) produced biofilm of which 28 (65%) were
strong producer 8 (18%) were moderate biofilm producer and 7(16.27%) were weak biofilm producers. All 43
isolates showed sensitivity for the mesenchymal stem cells with MIC range of 32-0.25µg.
Conclusion: So far only very few or no studies have been reported on anti-biofilm activity of mesenchymal
stems cells. From our study stem cell, therapy with MSC will be effective and alternate for antibiotic resistance
in chronic urinary infection there by can serve as therapeutic options for treating drug resistant organisms.
Biography
C Anitha has completed her PhD from Dr ALM PGIBMS, University of Madras, India. She is currently working as Assistant Professor of
Microbiology in Meenakshi Medical College Hospital and Research Institute. Her areas of interest are Biofilm studies using Confocal Laser
Scanning Microscope, Antimicrobial resistance and rare Infectious diseases. She has published more than 30 papers in reputed journals
and also has 15 Data sequences submitted in PUBMED/NCBI Genebank. She is also serving as an Editor, Associate Editor, Editorial Board
Member and Review Board Member of reputed journals.