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Volume 7 Journal of Infectious Diseases & Therapy
Page 44
Bacterial and Rare Diseases 2019
June 17-18, 2019
June 17-18, 2019 Dubai, UAE
Joint Meeting on
&
2nd Annual Congress on Bacterial, Viral and Infectious Diseases
6th International Conference on Rare Diseases & Orphan Drug
Senthamarai Thiyagarajan et al., J Infect Dis Ther 2019, Volume 7
Detection of mecA gene by PCR among coagulase negative Staphylococci from various
clinical samples in a tertiary care hospital
Senthamarai Thiyagarajan, C Anitha, S Sivasankari, K Muthulakshmi, V M Somasunder, K Akila, J Subha and Sijimol
Meenakshi Medical College Hospital and Research Institute, India
Introduction & Aim: Coagulase Negative Staphylococci (CoNS) are now emerging as important pathogen
and its resistance to antibiotics are worrisome. Methicillin resistance among CoNS causes an important
therapeutic threat associated with increased morbidity and mortality. This study was aimed to isolate the
coagulase negative Staphylococci from various clinical specimens, evaluate its antibiotic susceptibility pattern
and to detect the prevalence of mecA gene among coagulase negative Staphylococci.
Method: All the clinical samples were collected with aseptic precautions and processed as per standard
protocol. All the coagulase negative Staphylococcal isolates were subjected for antibiotic susceptibility testing
as per CLSI guidelines. Screening of methicillin resistance was done using cefoxitin disc (30 µg) as per CLSI
recommended disc diffusion method. Genotypic analysis for methicillin resistance (mecA gene) was done.
Results: A total of 89 clinically significant non-repetitive coagulase negative Staphylococcal isolates were
identified. S. epidermidis was most frequently isolated, among various species of CoNS. 15/89 (16.8%) were
methicillin resistant but only 9 CoNS isolates showed mecA genes.
Conclusion: Coagulase negative Staphylococci, which were previously dismissed as contaminants are now
emerging as important pathogen. There is necessity for continued surveillance to determine the extent of
emerging resistance in CoNS to reduce inappropriate use of antibiotics and to allow policies to be established
for adequate and rational use of antibiotics. This study insists the need of continuous monitoring the
prevalence of methicillin resistance among CoNS in a given population and to frame the antibiotic policy
because it can vary from region to region.
Biography
Senthamarai Thiyagarajan has completed her MD in Microbiology from Stanley Medical College. She is currently working as Professor in
Meenakshi Medical College Hospital and Research Institute, India. Her areas of interest are Mycology, HIV, Antibiotic Resistance, Infection
Control and Medical Education. She has received the Medical Excellence Award by Indian Solitarity Council at New Delhi and also has won
many best paper awards to her credit. She has published more than 30 articles in reputed journals.