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Volume 5, Issue 2 (Suppl) J Fisheries Livest Prod
ISSN: 2332-2608 JFLP, an open access journal Aquaculture Summit 2017
May 25-26, 2017
May 25-26, 2017 Osaka, Japan
6th Global Summit on
Aquaculture and Fisheries 2017
Purification, optimization and physicochemical properties of collagen from soft-shelled turtle calipash
Caiyan Li, Ya’nan Yang, Wei Song, Wei Wang and Guoying Qian
Zhejiang Wanli University, China
The present work was to optimize the purification conditions for soft-shelled turtle (Pelodiscus sinensis) calipash collagen (STCC)
isolated by pepsin and to explore collagen physicochemical properties for potential biomaterial applications. Single-factor test
and orthogonal method L9 (34) were employed with the STCC recovery yield as indicator. The optimum purification conditions were
obtained when NaCl concentration, collagen concentration and purification time were 2 M, 8 g/L, and 24 h, respectively. Purified
STCC were characterized by SDS-PAGE, UV scanning, FTIR, solubility, thermal behavior and amino acid analysis. The results showed
that STCC contained high hydroxyproline content than that of other fishery skins, belonging to typical type I collagen in form of [α1
(I)] 2α2 (I). FTIR spectra of STCC were quite similar to other aquatic animals’ collagens. It has the lowest solubility at pH 6, and when
NaCl concentration decreased from 2% to 6% (w/v), solubility dropped. The denaturation temperature (Td) and melting temperature
(Tm) were 35.1°C and 105.14°C, respectively. Morphology of STCC depicted as regular and porous network structure by SEM. In
general, the results suggested that turtle calipash can be exploited as alternatives to mammalian collagen and could also be used for
biomedical applications as a potential new material.
Biography
Research interest is to investigate the functional active substance and nutritional quality formation in aquatic animals, with emphasis on protein/bioactive peptide
preparation in Chinese soft-shelled turtles.
Caiyan Li et al., J Fisheries Livest Prod 2017, 5:2 (Suppl)