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Volume 5, Issue 2 (Suppl) J Fisheries Livest Prod
ISSN: 2332-2608 JFLP, an open access journal Aquaculture Summit 2017
May 25-26, 2017
May 25-26, 2017 Osaka, Japan
6th Global Summit on
Aquaculture and Fisheries 2017
Mr-Masc gene for sex-regulation and production of all-male populations in the giant freshwater prawn
Macrobrachium rosenbergii
Wen-ming Ma, Dong-rui Li, MS and Wei-jun Yang
Zhejiang Wanli University, China
Statement of the Background: Macrobrachium rosenbergii is one of the most important prawns in freshwater aquaculture. Male giant
freshwater prawns grow faster and reach higher weights at harvest than females, which make the culture of all-male populations
desirable. The purpose of this study is to explore the molecular information of new gene and its potential physiological function
on sex regulation. Methodology & Theoretical Orientation: The gene expression pattern was studied using Real-time RT-PCR. The
functional role of gene in vivo by RNA interference (RNAi) was utilized and its effect on sex regulation was evaluated. Findings: In
this study, a novel gene of the full-length cDNA encoding a masculinizing factor from the giant freshwater prawn Macrobrachium
rosenbergii was termed Mr-Masc. Real-time RT-PCR analysis revealed that Mr-Masc gene expression was obviously difference
between male and female prawn, and dominantly expressed in the ovary of the reproductive tract in female prawn. To elucidate
the functional role of Mr-Masc in vivo, the Mr-Masc gene was disrupted by RNA interference (RNAi). In vivo silencing of the gene,
by injecting the all-male and monosex postlarvae with Mr-Masc double-stranded RNA, sex-revsersed females (neofemales) were
obtained. Moreover, all-male progeny was produced by mating neofemales with normal males and these all-male populations showed
good growth potential and market value in the aquaculture. Conclusion & Significance: It was firstly reported the full and functional
sex reversal of male freshwater prawns through the silencing of a single Mr-Masc encoding gene. Neofemales capable of mating and
spawning were produced by the RNAi technology at a sufficiently early stage of larval development. This finding offered an insight
regarding the biology and evolution of sex differentiation regulation. Since sexual dimorphic growth patterns are common among
decapod crustaceans, it is obvious that the results of this study will have applied significance for many aqua cultured species.
Biography
Wen-ming Ma has expertise in sex determination and sex differentiation of the marine shrimp and prawn, functional gene discovery and application research.
Meanwhile, make a good progress in sex control and all-male unisexual cultivation in Macrobrachium rosenbergii.
Wen-ming Ma et al., J Fisheries Livest Prod 2017, 5:2 (Suppl)