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conferenceseries
.com
Volume 5, Issue 2 (Suppl)
J Fisheries Livest Prod
ISSN: 2332-2608 JFLP, an open access journal
Aquaculture Summit 2017
May 25-26, 2017
May 25-26, 2017 Osaka, Japan
6
th
Global Summit on
Aquaculture and Fisheries 2017
Microbial quality of tilapia tank water for produce production
Tung-Shi Huang and Jean Weese
Auburn University, USA
T
he microbiological quality of the water is important since the waste water from tilapia production tank will be used for produce
production. The USFDA Food Safety Modernization Act (FSMA) requirement of monitoring microbiological quality of produce
irrigation water,
Escherichia coli
and coliforms were analyzed. The water effluents from tilapia fish tank and greenhouse were
monitored twice a month. Since these vegetables, cucumbers and tomatoes, are used in ready-to-eat foods, there is a zero tolerance of
Salmonella
and
Listeria monocytogenes
. Therefore these two bacterial contents were examined. Membrane filtration and 3M Petrifilm®
were used to isolate
E. coli
and coliform in water samples.
E. coli
and coliform were also isolated from soil in green house for growing
produce using 3M Petrifilm and pour-plate methods. Enrichment protocol was used for
Salmonella
and
Listeria monocytogenes
detection for the cucumber and tomatoes. The population of
E. coli
from the effluent of tilapia tank in November, 2016 was higher,
then it decreased and the average population is 68 CFU/mL. The population of coliform has the same trend as in
E. coli
and the
average population is 1.2 x 10
3
CFU/mL. While the populations of
E. coli
and coliform from the effluent of greenhouse had the same
trend and the population of
E. coli
and coliform were 17 CFU/mL and 3.0 x 10
2
CFU/mL, respectively. The populations of
E. coli
and
coliform in the soil were 91 CFU/mL and 1.70 x 10
3
CFU/mL, respectively. For
Listeria monocytogenes
and
Salmonella
detection, one
positive of
Listeria monocytogenes
in presumptive test in cucumber has been found. The acquired data can provide information as to
whether further disinfection is needed before water is discharged into the environment.
Biography
He is awarded PhD in Food Science and Human Nutrition from University of Florida in the 1993. He holds a Master Degree (MSc) in Plant Pathology from National
Chung-Hsing University, Taiwan in the 1981, followed by a Bachelor’s Degree (BSc) in Plant pathology from National Chung-Hsing University, Taiwan 1978. He
has extended his/her valuable service as a
Professor
in Poultry Science, Auburn University for 6 years and has been a recipient of many award and grants. His
international experience includes various programs, contributions and participation in different countries for diverse fields of study. His research interests as a
Professor
reflect in his wide range of publications in various national and international journals.
huangtu@auburn.edu, weesesj@auburn.eduTung-Shi Huang et al., J Fisheries Livest Prod 2017, 5:2 (Suppl)
http://dx.doi.org/10.4172/2332-2608-C1-009