aquaculture-summit-2017-posters-accepted-abstracts

10 / 24

Page 65
Notes:
conferenceseries.com
Volume 5, Issue 2 (Suppl) J Fisheries Livest Prod
ISSN: 2332-2608 JFLP, an open access journal Aquaculture Summit 2017
May 25-26, 2017
May 25-26, 2017 Osaka, Japan
6th Global Summit on
Aquaculture and Fisheries 2017
Enhanced anti-inflammatory activity of brown seaweed Laminaria japonica by fermentation using Bacillus subtilis
Wen-Jung Lu1, Hong-Ting Victor Lin1, Guo-Jane Tsai1, Chien-Te Chou 1,2, Hsin-I Hsiao1 and Pai-A Hwang1
1National Taiwan Ocean University, Taiwan
2Genomics Research Center - Academia Sinica, Taiwan
Most studies on the anti-inflammatory effects of Laminaria japonica have investigated bioactive ingredients that have been
extracted from Laminaria using organic solvents. However, to avoid the toxicity and environmental issues caused by the solvent
used for extraction, increasing attention has focused on fermentation using microorganisms as a green bioconversion process for
producing bioactive compounds. In this study, fermentation with Bacillus subtilis, where the cultivation parameters and nutrient
additives were optimized to achieve the greatest inhibition of lipopolysaccharide (LPS)-induced nitric oxide (NO) production was
found to enhance the anti-inflammatory effects of L. japonica. Fermentation by B. subtilis using a 5% (w/v) L. japonica suspension as
the sole carbon source, 2% additional nitrogen source, a starting pH of 6, and incubation at 150 rpm at 37°C for 72 h yielded the best
anti-inflammatory effects, as LPS-induced NO production decreased to 27.6% ± 5.2%. The macrophages treated with the fermented
L. japonica product inhibited the LPS-induced production of reactive oxygen species (ROS) and NF-κB (p65) phosphorylation in a
concentration-dependent manner. Thus, our results show that L. japonica products generated by an optimized fermentation process
designed to enhance anti-inflammatory effects by decreasing ROS production, NF-κB (p65) phosphorylation, prostaglandin E2, and
NO production in macrophages.
Biography
Wen Jung Lu is a PhD student of National Taiwan Ocean University, Taiwan. Her topic is about the antibiotic resistance of microorganisms, and also doing the
bioactive compounds in algae. She has obtained the scholarship from National Science Council, Taiwan; the topic is ‘Bioactivity of beta-1, 3-xylan extracted from
seaweed by using beta-1, 3-xylanase express from E. coli. Moreover, she is working on the phycobiliproteins and also obtained the scholarship from the Haiden
Foundation, Taiwan. She is a competent researcher and she can work well with teams.
Wen-Jung Lu et al., J Fisheries Livest Prod 2017, 5:2 (Suppl)