aquaculture-summit-2017-posters-accepted-abstracts

11 / 24

Page 66
Notes:
conferenceseries.com
Volume 5, Issue 2 (Suppl) J Fisheries Livest Prod
ISSN: 2332-2608 JFLP, an open access journal Aquaculture Summit 2017
May 25-26, 2017
May 25-26, 2017 Osaka, Japan
6th Global Summit on
Aquaculture and Fisheries 2017
Hemoglobin from the blood clam Tegillarca granosa (Tg-HbIIA, Tg-HbIIB): Expression and antibacterial
activity of recombinant proteins
Yongbo Bao and Danli Song
Zhejiang Wanli University, China
The hemoglobins produced by Tegillarca granosa have antibacterial activity toward some Gram-positive and Gram-negative
bacteria. In this study, the genes encoding the recombinant proteins Tg-HbIIA and Tg-HbIIB were cloned from T. granosa
hemocytes by RT-PCR, and the proteins were expressed in Escherichia coli Transetta (DE3). The proteins were purified using a
HisTrap FF affinity chromatography column under denaturing conditions and refolded at 4 °C by urea gradient dialysis, and the
antibacterial activity of the recombinant proteins was determined. The Tg-HbIIA protein had antibacterial activity toward Vibrio
harveyi and Pseudomonas putida , with the minimum inhibition concentration (MIC) values of 6.58×10-2 mg/ml and 4.11×10-3 mg/ml,
respectively. The Tg-HbIIB protein had antibacterial activity toward V. harveyi, P. putida and Acinetobacter baumanii, withMIC values
of 1.58×10-1 mg/ml, 3.95×10-2 mg/ml and 7.90×10-2 mg/ml, respectively. They had no antibacterial activity against Staphylococcus
aureus, E. coli, Bacillus firmus, B. subtilis, S. epidermidis or V. parahaemolyticus . This study provides a basis for further research on the
antibacterial function and mechanism of hemoglobin.
Biography
Yongbo Bao has expertise in three field of his research: (1) Immune mechanism of molluscs: Molluscan immune and disease-resistant gene function, immune
mechanism, antibacterial peptides and antibacterial mechanism etc. (2) Mollucan genetics and molecular assistant breeding: Population genetic structure, genetic
linkage map, SNP and GWAS and phenotype association analysis. (3) Gene and molecular phylogeny and evolution: Immunity and development related gene
function study and molecular evolution analysis.
Yongbo Bao et al., J Fisheries Livest Prod 2017, 5:2 (Suppl)