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Journal of Biotechnology & Biomaterials | ISSN: 2155-952X | Volume: 8
July 23-24, 2018 | Vancouver, Canada
Annual Biotechnology Congress
In vitro
propagation response of
Rosmarinus officinalis
L. to biotic and abiotic elicitors on phenolic
content and photosynthetic pigments
Aisha Abdullah Alayafi
Jeddah University, Saudi Arabia
M
icropropagationprotocolwasmodified toevaluate thebest procedure to inducemultiple shoots from
Rosmarinus officinalis
L. sterilized seedlings obtained from seeds which vitality is always very low. Aseptic shoots (1-1.5 cm) were cultured on
full strength Murashige and Skoog medium modified with several growth regulators (abiotic elicitors): Benzyladenine (BA),
Kinetin (Kin) and coconut water (biotic elicitors). Data was indicated that BA at the concentration of 3 mg/L encouraged shoot
multiplication. The highest number of leaves and plant length also was obtained with medium modified with 3 mg/L BA and
coconut milk at 5 ml/L. All treatments were significantly different from the control. Total phenolics content, anthocyanins,
chlorophylls were extracted and spectrophotometrically determined as secondary products. Data was concluded the highest
content of phenolic 10.45 (mg/g) and chlorophyll b 0.67 (mg/g) when BA was 3.0 mg/L. Whereas chlorophyll a reached to 0.64
mg/g in presence of 5.0 mg/L BA and 5.0 ml/L coconut milk. Anthocyanin scored high level when BA was 3.0 mg/L combined
with 5.0 ml/L coconut milk.
Biography
Aisha Abdullah Alayafi is an assistant professor in Biology department and Faculty of Science from University of Jeddah, Saudi Arabia.
aal_shareaf@hotmail.comAisha Abdullah Alayafi, J Biotechnol Biomater 2018, Volume: 8
DOI: 10.4172/2155-952X-C3-095