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Journal of Biotechnology & Biomaterials | ISSN: 2155-952X | Volume: 8
July 23-24, 2018 | Vancouver, Canada
Annual Biotechnology Congress
Genome analysis of bio-control fungus
Trichoderma harzianum
and the biosynthesis of peptaibols
alamethicin
Jian Ling, Zhenchuan Mao
and
Bingyan Xie
Chinese Academy of Agricultural Sciences, China
T
richoderma harzianum
has strong ability for encoding secondary metabolites, which contribute a lot for its bio-control
function. peptaibols alamethicin is a family of antibiotics isolated from T. harzianum and was found significantly suppress
against various Fusarium fungi. Unfortunately, little was known about its biosynthetic genes and pathway to data. In this study,
we assembled the high-quality genome of
Trichoderma harzianum
T29, which consists of 72 scaffolds, with total size of 32 Mb
and the 1.5 Mb N50 value. A total of 10773 gene models were predicted in the genome. We predicted 36 secondary metabolite
gene clusters in T29 genome and the function of some secondary metabolite related genes were identified by homologous
deletion. We further identified a serial of genes from THBJ_06541 to THBJ _06568, including core enzymes, modifying
enzymes and transporter enzymes coded for the biosynthesis of alamethicin. To verify the biosynthesis pathway of peptaibols
alamethicin, we disrupted PKS (paD) in the cluster by homologous recombination and found the peptaibols alamethicin
were disappeared in Δ paD. These results will provide theoretical guidance for understanding the mechanism of
T. harzianum
against Fusarium fungi
.
Biography
Jian Ling has been engaged in the research of plant pathology in Institute of Vegetable and Flower Research in Chinese Academy of Agricultural Sciences (CAAS)
since 2008. His main researches are focused in analyzing the molecular mechanism of the interaction between crops and pathogens, using genomics and metab-
olomics, and developing the safe and efficient biocontrol technology against Fusarium wilt and root knot nematode.
lingjian2016@gmail.comJian Ling et al., J Biotechnol Biomater 2018, Volume: 8
DOI: 10.4172/2155-952X-C3-095