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Journal of Biotechnology & Biomaterials | ISSN: 2155-952X | Volume: 8
July 23-24, 2018 | Vancouver, Canada
Annual Biotechnology Congress
Optimized extraction and identification of functional
Orthosiphon stamineus
proteins
Kamal Usman
1
and
Zaidah Rahmat
2
1
Qatar University, Qatar
2
Universiti Teknologi Malaysia, Malaysia
T
he plant,
Orthosiphon stamineus
(cat whiskers) is a medicinal herb belonging to the family Lamiaceae, and the plant leaves
are commonly used as the herbal tea, popularly known as Java tea. It is used as a diuretic agent and for treatment against
heat rheumatism. Currently, there are no protein profile data available for this important plant species. This study focused
on the optimization of total protein extraction using four different extraction methods; QB buffer, phenol/sodium dodecyl
sulfate with three pre-washed steps, phenol/sodium dodecyl sulfate without pre-wash steps and sigma protein extraction kit,
it is aimed at determining the best protein extraction method, determine patterns and theoretically identify proteins. Overall,
phenol/sodiumdodecyl sulfate with three pre-wash steps result in better protein quality and pattern of separation. A total of 104
functional proteins were identified, each containing at least one unique peptide. Among the distinct proteins, rubisco activase
and triosephosphate isomerase correspond to the chloroplastic protein of photosynthesis/carbohydrate metabolism, while
phosphoglycerate kinase and glyceraldehyde are cytosolic enzymes of glycolysis pathway which were found to be significant
housekeeping proteins in of the leaf tissue. The result of this study will be useful for advanced pharmaceutical research and
could serve as a baseline for further proteomics work on
O. stamineus
and similar plant species.
Biography
Kamal Usman is an advanced level PhD candidate and currently working as a Teaching Assistant in the Department of Biological and Environmental Science at
Qatar University. He has a great passion for teaching and research. Since the completion of his undergraduate studies, and before joining Qatar University, not only
does he experienced classroom teaching and advanced level research exposure while completing his master’s degree at Universiti Teknologi Malaysia, was also
privileged to contribute to education’s development at the top level. He has served as a Senior Technical Officer on educational policy and development planning to
a UK funded project in Nigeria; state programme on accountability, responsiveness and capability (SPARC) between 2012 to 2013. He also briefly taught at Bayero
University, Kano preparatory to commence his PhD in 2015. His PhD thesis seeks to elucidate the mechanism of plants heavy metal uptake.
kusman@qu.edu.qaKamal Usman et al., J Biotechnol Biomater 2018, Volume: 8
DOI: 10.4172/2155-952X-C3-095