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Volume 08
Journal of Alzheimers Disease & Parkinsonism
Alzheimer's Congress 2018
May 30-31, 2018
May 30-31, 2018 Osaka, Japan
10
th
World Congress on
Alzheimer's Disease & Dementia
Neuritin attenuates early brain injury in rats after experimental subarachnoid hemorrhage
Dong Zhao
Shihezi University, China
Objectives:
Early Brain Injury (EBI) is central to the pathological progress of Subarachnoid Hemorrhage (SAH). In this study,
we determined if neuritin protects the brain against EBI in rats and discussed the role of apoptosis pathway mediated by
Endoplasmic Reticulum (ER) stress in this neuroprotective route.
Methods: A total of 96 male Sprague Dawley rats were divided into control, sham, SAH and SAH+neuritin groups. The rat
SAH model was induced by injection 0.3 mL of nonheparinized arterial blood into the prechiasmatic cistern. Mortality assay,
neurological scores, brain water content measurement, Evans blue dye assay, TUNEL stain assay, and Western blot analysis
were performed.
Results:
Neuritin significantly improved the neurological scores, brain water content, Blood Brain Barrier (BBB), and
apoptosis compared with the control and sham groups within 24 hours after SAH. TUNEL staining assay results demonstrated
that apoptosis was ameliorated, MMP-9 expression was reduced, whereas GRP78, CHOP, caspase-12, and ASK1 levels were
markedly preserved after neuritin application.
Conclusion:
Our study demonstrated that neuritin plays a neuroprotective role on EBI after SAH by attenuating BBB
disruption, brain edema and apoptosis.
zhanghang@stu.shzu.edu.cnJ Alzheimers Dis Parkinsonism 2018, Volume 8
DOI: 10.4172/2161-0460-C4-046