agri-world-2018-scientifictracks-abstracts

11 / 25

Page 40
Notes:
conferenceseries.com
11th World Congress on
March 05-07, 2018 | Paris, France
Plant Biotechnology and Agriculture
Volume 6 Advances in Crop Science and Technology
ISSN: 2329-8863 Agri World 2018
March 05-07, 2018
Protein baits, volatile compounds and irradiation influence expression profiling of odorant-binding protein
genes in Bactrocera dorsalis (Diptera: Tephritidae)
Atif Idrees, Hehe Zhang, Mijuan Luo, Min kyaw Thu, PumoCai, Waqar Islam, Jiahua Chen and Qinge Ji
Fujian Agriculture and Forestry University, China
C hemical communication among insects is based on odorant-binding proteins (OBPs). Regulatory mechanism of OBPs in the
perception of volatiles and host chemicals remain unclear. In present study, Bactrocera dorsalis OBPs (BdorOBPs) have been
evaluated in response to different attractive protein baits, brewer’s yeast volatile compounds and irradiation. Based on our previous
study, we analyzed the expression of 10 OBP genes expression in the antennae of B. dorsalis during three mature life-stages i.e. pre-
mating, post-mating and post-oviposition. B. dorsalis (Hendel) is one of the most economically important damaging fruit pests.
Selected BdorOBP genes were found with one conserved PBP-GOBP except OBP8 that contained two conserved domains. An un-
rooted phylogenetic tree was constructed to show the relationships among the 10 BdorOBPs to other insects having same OBP
family. We recorded significantly different transcriptome expression in each OBP genes at each stage in response to treatments. OBP2
was significantly expressed in response to baits at each developmental stage while OBP2, OBP5 and OBP1 were largely expressed
in response to combined volatiles treatments at all tested stages. OBP3, OBP5 and OBP10 showed high expression in response to
irradiation at all tested stages. We infer that our treatments have significant influence on the transcriptome of OBP genes which may
act in olfactory perception.
Biography
Atif Idrees is currently doing his PhD in Agriculture Entomology and Pests Management at Fujian Agriculture and Forestry University, China. He has done research
in physiology, entomology and ecology. His most recent publication is 'Genetic resistance in chickpea against Ascochyta blight: Historical efforts and recent
accomplishments'.
Atif Idrees et al., Adv Crop Sci Tech 2018, Volume 6
DOI: 10.4172/2329-8863-C1-005