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conferenceseries
.com
11
th
World Congress on
March 05-07, 2018 | Paris, France
Plant Biotechnology and Agriculture
Volume 6
Advances in Crop Science and Technology
ISSN: 2329-8863
Agri World 2018
March 05-07, 2018
Assessment of candidate DNA Barcoding loci for phylogenetic relationships among
Triticum
species
Lee Gi-An, Jung-Ro Lee, Gyu-Taek Cho, Kyung-Ho Ma
and
Sebastin Raveendar
National Agro-Biodiversity Center, Republic of Korea
Statement of the Problem
: DNA Barcoding is widely used for identification of species based on standard DNA regions. The
Consortium for the Barcode of Life (CBOL) plant-working group recommended different nuclear and chloroplast loci as the
standard plant barcode. Though many loci have been proposed as a DNA barcodes in plants, standardizing regions as a DNA
barcode poses a challenge in many plant families. The evolutions of the chloroplast regions combine with nuclear gens are
sufficiently rapid to allow discrimination between closely related species.
Material & Methods
: In this study, we tested the phylogenetic utility of the DNA barcoding loci (ITS2, matK, psbA-trnH,
rbcL and trnT) for efficient discrimination of
species. For the assessment of the barcoding efficiency to resolve the
species discrimination, a total of 113 accessions representing 18 species/sub-species within
Triticum
genus have been sampled.
Consensus sequences of each region were manually edited with MEGA6 and the sequences were aligned and assessed for
candidate DNA Barcode for phylogenetic relationships among
Triticum
species.
Findings:
Topologies of the phylogenetic trees based on combination of DNA barcode analyses were similar but a few
Triticum
species were placed into distant phylogenetic groups. The 113 accessions analyzed in this study were placed into three groups
supported by high bootstrap values. However, the barcoding analyses were not able to discriminate some closely related
Triticum
species.
Conclusion & Significance
: We have proposed concatenated data approach to increase resolving power of candidate barcoding
loci as an additional tool for phylogenetic analysis in
Triticum
species. However, molecular studies with more diverse markers
and species will be required to clarify the ambiguities surrounding the phylogeny of these important genera.
Biography
Lee Gi-An has his expertise in evaluation and characterization of plant genetic resources. He has worked in National Agro-Biodiversity Center (NAc, RDA, Republic
of Korea) for more 12 years as a Researcher.
gkntl1@korea.krLee Gi-An et al., Adv Crop Sci Tech 2018, Volume 6
DOI: 10.4172/2329-8863-C1-006