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conferenceseries.com
11th World Congress on
March 05-07, 2018 | Paris, France
Plant Biotechnology and Agriculture
Volume 6 Advances in Crop Science and Technology
ISSN: 2329-8863 Agri World 2018
March 05-07, 2018
Loss of function of SPFF, a novel receptor-like kinase, induces parthenocarpy in tomato
Hitomi Takei1, Yoshihito Shinozaki1, Ryoichi Yano1, Hiroshi Ezura1, Tohru Ariizumi1, Michel Hernould2 and Christian Chevalier2
1University of Tsukuba, Japan
2INRA, France
We are facing to the global warming. Fruit yield is deceased under high temperature because of a failure in pollination.
To improve fruit yield under harsh environmental conditions, it is needed to understand the mechanism of fruit set.
However, its genetic and molecular factors remain poorly understood. Analyzing the mechanism of parthenocarpy, fruit set
without pollination, will help to identify the key regulators that control fruit set. A mutant named small parthenocarpic fruit
and flower (SPFF) was obtained by γ-ray irradiation of Micro-Tom seeds. In this study, we aimed to identify and characterize
the responsible gene for parthenocarpy in SPFF mutant. First, we characterized the visible phenotypes, size of cells and ploidy
levels. The characterization of SPFF mutant was male sterility, floral organs dwarfism and parthenocarpic fruits with high
ploidy levels. Second, in order to identify the responsible gene for these phenotypes, positional cloning by fine mapping and
RNAi strategy were performed. They allowed us to identify that a loss of function of a gene coding a receptor like kinase
(SPFF) triggers parthenocarpy. Third, to analyze the function and characterization of SPFF gene, RNA sequence and in situ
hybridization were performed. SPFF expressed higher at receptacle than the other organs including reproductive organs in
developing buds. The expression level of cell cycle genes (CDKB) and the gene maintaining stem cell (WUSCHEL) were
affected at developing ovule. According to these results, our research brought a new point of view of the mechanism of fruit
set, that they are supposed to be regulated by a receptor like kinase named SPFF. Furthermore, we also brought an idea that a
responsible gene for parthenocarpy was expressed in receptacle and supposed to be in vascular bundle although the function
of receptacle and vascular bundle during fruit set in tomato had not been noticed so far.
Biography
Hitomi Takei has received Bachelor’s degree of Bio-resource Science from the University of Tsukuba in 2016. Currently she is pursuing International Double degree
Master’s Program co-organized by the University of Tsukuba in Japan and University of Bordeaux in France. She has her passion in working toward a solution of
food security problems in African countries and other developing regions of the world by improving yields of vegetables and crops.
Hitomi Takei et al., Adv Crop Sci Tech 2018, Volume 6
DOI: 10.4172/2329-8863-C1-006