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Addiction Therapy 2016
October 03-05, 2016
Volume 7, Issue 5(Suppl) J Addict Res Ther
ISSN:2155-6105 JART, an open access journal
conferenceseries .com
October 03-05, 2016 Atlanta, USA
5th International Conference and Exhibition on
Addiction Research & Therapy
Jes rejoice Paul, J Addict Res Ther 2016, 7:5(Suppl)
Dopamine D1 and D2 receptor subtypes functional regulation in unilateral rotenone lesioned parkinson’s rat
model: effect of serotonin, dopamine and norepinephrine
Jes rejoice Paul
American University of St Vincent, USA
Parkinson’s disease (PD) is due to widespread degeneration in the central and peripheral nervous systems. The hallmark
pathology remains in the dopaminergic striatal insufficiency and degeneration of dopaminergic neurons in the substantia
nigra (SN).
Objectives: - The present study analysed the effect of serotonin (5-HT), dopamine and norepinephrine (NE) as treatment on
rotenone induced hemi-Parkinson’s disease in rats and its role in the regulation of Dopamine receptor subtypes in the Corpus
Straiatum (CS) of the experimental rats.
Methods: - Unilateral stereotaxic single dose infusions of rotenone were administered to the substantia nigra of adult male
Wistar rats. Neurotransmitters –serotonin (5-HT), dopamine and norepinephrine (NE) treatments were given to rotenone
induced hemi-Parkinson’s rats. Dopamine receptor and its subtypes (D1 and D2) binding assay were done. Gene expression
studies of Dopamine D1 and D2 were done using real-time PCR.
Results: - Scatchard analysis of Dopamine and Dopamine D2 receptor showed a significant increase (p<0.001) and Dopamine
D1 receptor showed a significant decrease (p<0.001) in the Bmax in Corpus Striatum of the PD rats compared to control. These
altered parameters were reversed to near control in the serotonin and norepinephrine treated Parkinson’s disease rats and no
change was observed in Dopamine treated Parkinson’s disease rats. Real-time PCR results confirmed the receptor data.
Conclusion: - Our results showed serotonin and norepinephrine functionally reversed in Dopamine receptors in rotenone
induced hemi-Parkinson’s rat. This has clinical significance in the therapeutic management of Parkinson’s disease.
Key words: Corpus Striatum; Dopamine D1 and D2 receptor subtypes; Parkinson’s disease; Rotenone; Substantia nigra pars
compacta.
Biography
Jes Paul is doing research on Stem Cell, Genetics, Medical Biochemistry, pathophysiology, Molecular and Cellular neurobiology. He worked as an Associate
Research Scientist Texas Tech University Health Sciences Center. He did Post-doctoral researcher from Ohio State University Medical Center.