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Volume 4, Issue 3 (Suppl) Adv Crop Sci Tech
ISSN: 2329-8863 ACST, an open access journal
Page 50
Notes:
Plant Genomics 2016
July 14-15, 2016
conferenceseries.com
July 14-15, 2016 Brisbane, Australia
4th International Conference on Plant Genomics
Drought tolerance mediated by the rice ubiquitin ligase OsDt1
Wen-Yuan Song, Xiaoen Huang, Xiuhua Chen, Sisi Gang and Sixue Chen
University of Florida, USA
Drought stress is a major limitation to crop production in the worldwide. We have identified ubiquitin ligase OsDt1 that can
aid drought survival of rice. The OsDt1 gene is strongly induced by water deficit. While down-regulation increases drought
sensitivity, overexpression of OsDt1 allows plants to confer strong tolerance to water stress. RNA-seq assays identified a total of 307
differentially expressed genes (DEGs) between the OsDt1 overexpression line OXDt1-70 and the empty-vector control line A36
under normal conditions. Among them, 24% (50 up-regulated and 24 down-regulated) DEGs have been reported previously to be
associated with water stress or drought regulators. The OsDt1 protein localizes to both the plasma membrane and nucleus in rice
cells. Protein blot analysis suggests that the ubiquitin ligase activity of OsDt1 might be active in the nuclear pools. Yeast two-hybrid
analysis shows that OsDt1 interacts with a negative regulator of drought response. These results suggest that OsDt1 function as a key
regulator of drought survival in rice.
Biography
Wen-Yuan Song has completed his PhD in 1995 from the Institute of Genetics, Chinese Academy of Sciences, China and Postdoctoral studies from the University
of California-Davis, USA. He is currently an Associate Professor at the University of Florida, USA. His research mainly focuses on plant response to biotic and
abiotic stresses.
Wen-Yuan Song et al., Adv Crop Sci Tech 2016, 4:3 (Suppl)