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Volume 4, Issue 3 (Suppl) Adv Crop Sci Tech
ISSN: 2329-8863 ACST, an open access journal
Page 48
Notes:
Plant Genomics 2016
July 14-15, 2016
conferenceseries.com
July 14-15, 2016 Brisbane, Australia
4th International Conference on Plant Genomics
C2Pe: Anew open source qPCR algorithm and web interface for raw data analysis
Juan Pablo Matte1, J Padarian2 , R Siqueira3, N Wilson2 and B Jones2
1Pontificia Universidad Catolica de Chile, Chile
2The University of Sydney, Australia
3University of Lausanne, Switzerland
The determination of transcript levels via quantitative polymerase chain reaction (qPCR) is now widespread and instrumental in
research, medicine and a broad range of other areas. The standard method of analysis is delta Ct, that is simple to use, but has a
high probability of deviating from the true value. Several alternative approaches that give more consistent results have been presented
but they are all complex to use. The C2Pe method we have developed can improve on the delta Ct method by 51%, using the PCR
curve to estimate the efficiency of each reaction. The main innovations are that the embedded equations use the second phase of the
curve to add a quality value to the data to correct the information given by the exponential phase. We have also incorporated revised
statistics to improve the robustness of the analysis and constructed a web-based interface to give a straightforward, streamlined qPCR
experience.
Biography
Juan Pablo Matte has studied both, Forestry Engineering and Biological Science degree in the Pontificia Universidad Catolica de Chile. He has completed his PhD
in 2013 and Postdoctoral studies in 2015 from The University of Sydney, Australia. Currently he is an Associate Researcher at the Pontificia Universidad Catolica
de Chile, under the PAI project number 82140040 from CONICYT.
Juan Pablo Matte et al., Adv Crop Sci Tech 2016, 4:3 (Suppl)