Plant-Genomics-2016_Scientific-Tracks-Workshop

21 / 60

Volume 4, Issue 3 (Suppl) Adv Crop Sci Tech
ISSN: 2329-8863 ACST, an open access journal
Page 47
Notes:
Plant Genomics 2016
July 14-15, 2016
conferenceseries.com
July 14-15, 2016 Brisbane, Australia
4th International Conference on Plant Genomics
Application of next-generation sequencing, genome sequencing and whole genome re-sequencing to
practical plant breeding: Case studies on lupin
Huaan Yang
Department of Agriculture and Food Western Australia, Australia
Next-generation sequencing (NGS) and whole genome sequencing bring us a deluge of genome and genetic variations. In lupin,
we are applying NGS and genome sequencing technologies to help practical plant breeding through four avenues. (1) NGS as
DNA fingerprinting for rapid trait-marker association discovery. Markers closely linked to disease resistance genes were successfully
discovered by NGS-based RAD sequencing and were applied to marker assisted selection (MAS) in lupin. (2) Genome sequencing for
developing functional markers. We completed the draft genome sequence of lupin; a diagnostic marker linked to anthracnose disease
resistance gene was developed by gene annotation and further confirmed by genetic mapping. (3) Genome sequencing to developed
cost-effective markers. In the last 15 years, DAFWA has developed over a dozen of gel-based InDel markers linked to various genes
of agronomic traits of interest in lupin. Using the draft lupin genome sequence as templates, all previously developed InDel markers
were converted into a cost-effective SNP markers to suit modern SNP genotyping platform. (4) Whole genome re-sequencing for
developing diagnostic markers for MAS. We recently resequenced the whole genomes of17 lupin lines; several million markers were
documented and 207,887 markers were anchored on the lupin genetic linkage map. We demonstrated two protocols of using whole-
genome resequencing data for rapid development of diagnostic markers for MAS. In conclusion, the cases in lupin represent one of a
few successful cases where NGS and genomes sequencing have been used as routine tools for molecular plant.
Biography
Huaan Yang is a Plant Pathologist by training with BSc in 1982 and MSc in 1985. He has completed his PhD from University of Western Australia in 1992. He
became a Molecular Geneticist following his development of a DNA fingerprinting technology called “MFLP” in 2001. His lab has been the only lab for large-scale
application molecular markers on MAS in plant breeding on legume grain crops in Australia since 2004. Since 2011, his lab has been fully switched into NGS and
genome sequencing based approaches for pre-breeding in lupin.
Huaan Yang, Adv Crop Sci Tech 2016, 4:3 (Suppl)