infectious-diseases-2016_posters-accepted-abstracts

8 / 30

Volume 4, Issue 4(Suppl) J Infect Dis Ther 2016
ISSN: 2332-0877, JIDT an open access journal
Page 90
Notes:
Infectious Diseases 2016
August 24-26, 2016
conferenceseries.com
August 24-26, 2016 Philadelphia, USA
&
Infectious DiseasesJoint Event on 2nd World Congress on
Pediatric Care & Pediatric Infectious Diseases
International Conference on
In vitro anti-amoebic potential of medium chain triglycerides compounds
Nurul Azira Binti Mohd Shah1, Wan Nor Amilah Wan Abdul Wahab2, Siti Farah Alwani Mohd Nawi1, Baha Latif1, Zaini Mohd Zain 1, Rosliza Suhaimi1 and Nazli
Zainuddin1
1Universiti Teknologi MARA, Malaysia
2Universiti Sains Malaysia, Malaysia
Background: Amoebiasis is a parasitic disease caused by Entamoeba histolytica that causes death in developing country. The gold
standard treatment for amoebiasis is metronidazole. However, the emergence of resistant against metronidazole has been increasingly
reported. Thus, it is important to search for other alternative control measure which is safe and effective. Medium chain triglyceride
(MCT), an active compound in natural products is widely available as a nutrient supplement and has been shown to have anti-
protozoal properties.
Objective: Therefore the objective of this study is to investigate the potential effect of medium chain triglycerides (MCT) against
amoebic infections.
Methods: The trophozoites of Entamoeba histolytica HM1: IMSS strain were axenically cultivated in TYI-S-33 medium in culture
flasks and incubated anaerobically by using Anaerocult A (Merck). The anti-amoebic effect was determined by measuring the
minimum inhibitory concentration (MIC) of E. histolytica against MCT active compounds which include lauric acid, capric acid,
caprylic acid and caproic acid were based on scoring of trophozoites growth using the standard method.
Results: Lauric acid and capric acid exhibited anti-amoebic effect with minimum inhibitory concentration (MIC) of 400 μg per ml
and 800 μg per ml respectively.
Conclusion: In conclusion, MCTs was active against E. histolytica in vitro and should be considered as a probable anti-amoebic
compound in amoebiasis.
Biography
Nurul Azira Binti Mohd Shah has completed her degree in Bachelor of Medicine and Bachelor of Surgery (MBBS) from University of Malaya, Malaysia. She was also
awarded Master of Pathology (MPath) in Clinical Microbiology from Universiti Sains Malaysia. She is a Clinical Microbiologist and Medical Lecturer in Universiti Teknologi
MARA, Malaysia.
Nurul Azira Binti Mohd Shah et al., J Infect Dis Ther 2016, 4:4(Suppl)