infectious-diseases-2016_posters-accepted-abstracts

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Volume 4, Issue 4(Suppl) J Infect Dis Ther 2016
ISSN: 2332-0877, JIDT an open access journal
Page 88
Notes:
Infectious Diseases 2016
August 24-26, 2016
conferenceseries.com
August 24-26, 2016 Philadelphia, USA
&
Infectious DiseasesJoint Event on 2nd World Congress on
Pediatric Care & Pediatric Infectious Diseases
International Conference on
Detection and quantification of pro-inflammatory cytokine in sera and urine of Sudanese patients
infected with Schistosoma haematobium
Musa H A, Hammad A, Elfadil A G, Osman M A, El Safy H S, Fayed O M, Bashir A, Mustafa A and Alfarazdeg A
1National Ribat University, Sudan
2University of Becht Elruda, Sudan
3University of Khartoum, Sudan
Background: Schistosome-specific acquired immunity capable of reducing levels of infection or re-infection develops slowly. The
nature of these protective immune responses suggesting that anti-helminths immune responses fall into a Th1 (pro-inflammatory)
and Th2 (anti-inflammatory).
Objective: The aim of this study was the detection and quantification of pro-inflammatory cytokines in both sera and urine of
patients with urinary schistosomiasis.
Methodology: One hundred and thirty sera and urine were collected from patients with urinary schistosomiasis in two villages south
Elduiem (Sudan). The disease was confirmed by finding Schistosoma haematobium eggs in urine using syringe filtration techniques.
To rule out Schistosoma mansoni and other helminths infection Kato Katz technique for stool examination was used. Sera and urine
of seventy subjects who were Schistosoma haematobium negative included in the study as controls. Samples were examined for IFNγ
and IL-2, cytokines level using ELISA techniques.
Results: The prevalence of Schistosoma haematobium infection in the study population showed that the peak of infection was in
childhood (3-13 years) followed by a sharp decline in infection intensity. Males infected more than females 62% and 38% respectively.
Measurements of parasite specific cytokine responses showed high levels of both cytokine productions before treatment then after
treatment. There are significant differences in cytokine production in infected groups and control one. Females produce high levels
of IFNγ and IL-2 than males. Aged people produce high levels of both cytokine than young people. There are a positive association
between the production of cytokine and intensity of the infection.
Conclusion: The conclusion drawn from the study is that pro-inflammatory cytokines were produced in large amount before
treatment with significant difference between males and females.
Biography
Musa H A is affiliated to National Ribat University, Sudan
Musa H A et al., J Infect Dis Ther 2016, 4:4(Suppl)