infectious-diseases-2016_posters-accepted-abstracts

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Volume 4, Issue 4(Suppl) J Infect Dis Ther 2016
ISSN: 2332-0877, JIDT an open access journal
Page 104
Notes:
Infectious Diseases 2016
August 24-26, 2016
conferenceseries.com
August 24-26, 2016 Philadelphia, USA
&
Infectious DiseasesJoint Event on 2nd World Congress on
Pediatric Care & Pediatric Infectious Diseases
International Conference on
Clonal spread of Staphylococcus aureus isolates with reduced sensitivity to chlorhexidine in Kuwaiti
hospitals
Leila Vali, Ali A Dashti and Febine Mathew
Kuwait University, Kuwait
It is essential to reduce the bacterial load and ensure the effectiveness of hygienic conditions in hospitals. Chlorhexidine is one
of the most widely used biocides. Since resistance genes to quaternary ammonium compounds have appeared more frequently
in staphylococci, we aimed to identify S. aureus with reduced sensitivity to Chlorhexidine in Kuwaiti hospitals. We collected 97
methicillin-resistant (MRSA) and 100 methicillin-sensitive (MSSA) S. aureus. We amplified mecA, blaZ, qac A/B, qacH, qacH2, qacG,
smr, norA, PVL and agr genes. Antibiotic sensitivity testing for 10 antibiotics and MIC/MBC of chlorhexidine were determined.
MLST, spa typing and PFGE were used to identify genetic variations. The predominant genes were mecA, blaZ, norA, qacA/B, smr,
while qacG, qacH, qacH2 were not detected. All isolates were sensitive to vancomycin, teicoplanin and tigecycline. The range of MIC
for chlorhexidine for qacA/B positive isolates was 4-30 mg/l, however the MBC (0.94-60 mg/l) was statistically greater than MIC
(p<0.0001). Typing methods showed all the isolates with reduced sensitivity to chlorhexidine containing qacA were ST239-III-t945-
PVL_. The presence of qac genes does not necessarily imply increased resistance to chlorhexidine or any other antimicrobial agents.
However MBC of chlorhexidine was higher for the isolates containing qacA/B compared to those without. We observed clonality
among the qacA/B positive isolates with reduced sensitivity to chlorhexidine (HA-MRSA-ST239-III-t945 PVL_). Reduced sensitivity
may be a contributing factor to survival of this clone in hospitals. It is crucial to strictly monitor dilution standards to avoid low level
exposure of HA-MRSA-ST239-III to biocides, specifically in the developing countries.
Biography
Leila Vali has completed her PhD from Edinburgh University and Postdoctoral studies from Medical College of Wisconsin, Milwaukee and Edinburgh University. She has
been a Lecturer in Strathclyde University, UK and is currently an Assistant Professor in Kuwait University. He has published more than 25 papers in reputed journals and
has been serving as an Editorial Board Member of repute.
leila@hsc
Leila Vali et al., J Infect Dis Ther 2016, 4:4(Suppl)