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Volume 4, Issue 5(Suppl) J Infect Dis Ther
ISSN: 2332-0877 JIDT, an open access journal
Page 15
Euro Infectious Diseases 2016
September 05-06, 2016
conferenceseries.com
Infectious Diseases
September 05-06, 2016 Frankfurt, Germany
3rd Euro-Global Conference on
Identification and differentiation of the most clinically involved Candida species in neonates with
candidemia admitted to NICU in Cairo University Specialized Pediatric Hospital (CUSPH)
Mona Mohiedden and Heba Abo Hussein
Cairo University, Egypt
Background: Candidemia studies have documented geographic differences in rates and epidemiology. Although Candida albicans
continues to be the most common and virulent cause of Candida blood stream infection (BSI), longitudinal studies have detected an
increase in the incidence of BSI caused by other Candida species that are known to be inherently less susceptible to commonly used
antifungal drugs.
Study Question: Is to investigate the new trend of neonatal candidemia due to most commonly encountered Candida species using
simple and reliable technique.
Methods: Blood cultures were performed in BACTEC instrument for 107 neonates admitted to Neonatal Intensive Care Unit, Cairo
University Specialized Pediatric Hospital (CUSPH). All study population was suffering from prolonged hospitalization with fever
of unknown origin, inadequate antibiotic response for at least one week. Detection of candidemia and species identification of
isolates was performed according to its standard protocol. All blood culture bottles of the Candida isolates and bottles that did not
flag positive and gives negative subculture on Sabaraud dextrose agar (SDA) and sheep blood agar (SBA) 5th day of incubation in
BACTEC instrument were further identified and differentiated using PCR technique. The non-systematic collection and storage of
samples were a limitation of our study.
Results: 98 (91.6%) out of 107 studied cases were culture positive for fungus. 90 of these cases (91.8%) were PCR positive, while 8
cases (8.16%) were not identified by PCR. The agreement between the two techniques was 0.229 (P value=0.017). The designated
inner primers for the given Candida species identified all 96 cases to species level were 70/107 (65.4%) as Candida albicans, 14/107
(13.1%) as Candida tropicalis, 12/107 (11.2 %) as Candida glabrata. All cases that showed positive Germ tube test 45/98 (45.9%) were
confirmed as Candida albicans by PCR. There was no statistically significant difference between identified Candida species in the
present study regarding clinical diagnosis or demographic criteria.
Conclusions: Notably we have performed a reliable technique for comprehensive identification of clinically relevant Candida isolates
and ascertained significant data on many technical points including; specimen type, time and storage conditions. Performing a
study for evaluation of the effect of different blood fractions on the reproducibility of PCR results for diagnosis of candidemia is
recommended.
Public Health Implications: PCR technique is more specific and rapid than conventional culture method. Moreover, the improved
detection and discrimination between infecting Candida species is additional advantage information that is crucial for initiating
specific antifungal therapy.
Biography
Prof. Dr. Mona Mohiedden Abdel Halim completed her PhD at the age of 31 years from Faculty of medicine-Cairo University. She completed her postdoctoral
studies in clinical microbiology from Cairo University School of Medicine. Currently, she is the director of microbiology unit of the main laboratory of Cairo University
Specialized, pediatric hospital (CUSPH). She is also leader of infection control of same CUSPH hospital since about 8 years. Dr. Mona has published more than
20 papers in the field of microbiology and infection control in reputed journals and attended more than 30 national and international conferences and workshops in
field of microbiology and infection control as speaker, organizer and poster presenter. She is member of various Professional Associations..
• National: Member of ESLM
• International: Associate member of international federation of infection Control.
• Reviewer in some scientific journals
Mona Mohiedden et al., J Infect Dis Ther 2016, 4:5(Suppl)