euro-infectious-diseases-2016_scientifictracks-abstracts

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Volume 4, Issue 5(Suppl) J Infect Dis Ther
ISSN: 2332-0877 JIDT, an open access journal
Page 14
Notes:
Euro Infectious Diseases 2016
September 05-06, 2016
conferenceseries.com
Infectious Diseases
September 05-06, 2016 Frankfurt, Germany
3rd Euro-Global Conference on
Electron microscopic assay of bacterial biofilm formed on indwelling urethral catheters
Nada Nabil Nawar
Cairo University, Egypt
Biofilm formation in indwelling urinary catheters is a leading cause of urinary tract infection (UTI) that is associated with
increased bacterial resistance to antimicrobial therapy and treatment failure. Our aim was to find out the incidence of biofilm
formation in urinary catheterized patients and to detect a reliable diagnostic technique for the detection of the formed biofilm.
The study was done on 20 hospitalized patients from Cairo University Hospitals with indwelling catheters for ≥3 days that were
scanned by electron microscopy (SEM) for the presence of biofilm. Microorganisms isolated from catheter and urine samples were
identified and tested for biofilm formation using the tissue culture plate (TCP). The microbial species isolated from cultured urine
were E. coli, Acinetobacter, Candida albicans and non -C. albicans. Biofilm formation increased with duration of catheter in situ, but
no significant correlation was found (p=0.095). There was reduction in microbial diversity with antimicrobial use but the correlation
was insignificant (p=0.317). SEM images of 14/20 (70%) showed biofilms on the luminal surface. The sensitivity, specificity, total
accuracy, PPV and NPV of urine culture and catheter culture against SEM were 64.30%, 100%, 75%, 100%, 54%, 85.70%, 100%, 90%,
100%, 75% respectively. Combined routine microbiological examination of urine and intraluminal catheter surface is required for
early identification of organisms causing catheter associated urinary tract infections (CAUTI) and biofilms on urinary catheters. SEM
proved to be a reliable method for the detection of biofilm on urinary catheters than urine culture and TCP assay.
Biography
Nada Nabil Nawar has completed her PhD from Kasr Al Aini School of Medicine, Cairo University, Egypt. She is currently working as a Professor of Clinical Pathology in
Cairo University, Egypt. She has more than 18 papers in reputed journals.
Nada Nabil Nawar, J Infect Dis Ther 2016, 4:5(Suppl)