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conferenceseries.com
Volume 9, Issue 9 (Suppl) J Cancer Sci Ther, an open access journal
ISSN: 1948-5956 World Cancer 2017
October 19-21, 2017
25th WORLD CANCER CONFERENCE
October 19-21, 2017 | Rome, Italy
Stroma-derived extracellular vesicles deliver tumor-suppressive mi-RNAs to pancreatic cancer cells
Song Han
University of Florida, USA
The biology of tumor-associated stroma (TAS) in pancreatic ductal adenocarcinoma (PDAC) is not well understood. The
paradoxical observation that stroma-depletion strategies lead to progression of PDAC reinforced the need to critically
evaluate the functional contribution of TAS in the initiation and progression of PDAC. PDAC and TAS cells are unique in their
expression of specific miRNAs, and this specific miRNA expression pattern alters host to tumor microenvironment interactions.
Using primary human pancreatic TAS cells and primary xenograft PDAC cells co-culture, we provided evidence of miRNA
trafficking and exchanging between TAS and PDAC cells, in a two-way, cell-contact independent fashion, via extracellular
vesicles (EVs) transportation. Selective packaging of miRNAs into EVs led to enrichment of stromal specific miR-145 in EVs
secreted by TAS cells. Highly-concentrated exosomes, but not micro-vesicles, derived from human TAS cells demonstrated
a tumor suppressive role by inducing PDAC cell apoptosis. This effect was mitigated by anti-miR-145 sequences. Our data
suggest that TAS-derived miRNAs are delivered to adjacent PDAC cells via exosomes and suppress tumor cell growth. These
data highlight that TAS cells secrete exosomes carrying tumor suppressive genetic materials, a possible anti-tumor capacity.
Future work of the development of patient-derived exosomes could have therapeutic implications for unresectable PDAC.
Biography
Song Han completed her MD in 1987 and PhD in 1996, both from Shanghai Jiao Tong University School of Medicine, China. She received Postdoctoral training
(1996-2008) at Cardiff University, UK. She is currently a Research Faculty at the University of Florida. Her research interest includes the understanding and
translational application of extracellular vesicle transporting microRNAs in the cross-talk between tumor-associated stroma (TAS) and cancer cells in pancreatic
cancer tumor microenvironment. She has published more than 45 papers in reputed journals and has been serving as reviewer of several high-impact journals.
Song Han, J Cancer Sci Ther 2017, 9:9(Suppl)
DOI: 10.4172/1948-5956-C1-111