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conferenceseries.com
Volume 9, Issue 9 (Suppl) J Cancer Sci Ther, an open access journal
ISSN: 1948-5956 World Cancer 2017
October 19-21, 2017
25th WORLD CANCER CONFERENCE
October 19-21, 2017 | Rome, Italy
J Cancer Sci Ther 2017, 9:9(Suppl)
DOI: 10.4172/1948-5956-C1-112
Why extracts of five Indian plants cure cancer: Enhanced protection of DNA but destruction of
nucleotides through the endogenous fenton reaction, and inhibition of human topoisomerases
Rajagopal Chattopadhyaya and Indrani Kar
Bose Institute, India
The influence of substoichiometric amounts of seven plant extracts in the Fenton reaction-mediated damage to
deoxynucleosides, dNMPs, dNTPs and supercoiled plasmid DNA were studied to rationalize anticancer properties
reported in the extracts of Acacia catechu, Emblica officinalis, Spondias dulcis, Terminalia belerica, Terminalia chebula. Extracts
from these five plants, as well as four pure compounds contained, enhance the extent of damage in Fenton reactions with
all monomeric substrates but protect supercoiled plasmid DNA, compared to standard Fenton reactions. However, Dolichos
biflorus and Hemidesmus indicus extracts generally do not show this enhancement for the monomeric substrates though they
protect plasmid DNA. A catalytic mechanism involving the presence of a ternary complex of the nucleoside / nucleotide
substrate, a plant compound and the hydroxyl radical was proposed [J. Biomol. Struct. Dyn. 2016; doi:10.1080/07391102.2016.
1244493]. Such a mechanism cannot operate for plasmid DNA. These plant extracts will slow down DNA replication in rapidly
dividing cancer cells. In another set of experiments, extracts of the same five plants completely inhibit human topoisomerase
I at 40 μg/ml concentration while Hemidesmus indicus and Dolichos biflorus extracts inhibit partially. All seven plant extracts
partially inhibit human topoisomerase II at 120 μg/ml concentration. Chebulagic and chebulinic acids purified from Terminalia
chebula extract inhibited human topoisomerase I at around 2 μM and 3 μM respectively [Molecular Enzymology and Drug
Targets 2017. Vol. 2. No. 2. ]. The nuclear fragmentation leading to apoptosis observed earlier in
cancerous cell lines with such plant extracts may thus be explained by the inhibition of topoisomerases.