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conferenceseries
.com
Volume 9, Issue 9 (Suppl)
J Cancer Sci Ther, an open access journal
ISSN: 1948-5956
World Cancer 2017
October 19-21, 2017
25
th
WORLD CANCER CONFERENCE
October 19-21, 2017 | Rome, Italy
J Cancer Sci Ther 2017, 9:9(Suppl)
DOI: 10.4172/1948-5956-C1-112
Studies on anti-neoplastic enzyme: enhanced L-asparaginase activity by filamentous fungus from the
Brazilian Savanna using Plackett-Burman Design for screening of culture medium variables
Pérola Magalhães
1
, Marcela Medeiros de Freitas
1
, Paula Monteiro Souza
1
, Samuel Cardoso
1
, Edilvaldo Ximenes
1
and
Adalberto Pessoa
2
1
University of Brasília, Campus Darcy Ribeiro, Brazil
2
University of São Paulo, Brazil
L
-asparaginase is an enzyme used for treatment of Acute Lymphoblastic Leukemia (ALL) in children. Neoplastic cells
cannot synthesize L-asparagine unlike normal cells due the absence of L-asparagine synthetase; therefore they obtain the
required asparagine from circulating pools. It is important to find new sources of Lasparaginase producing microorganisms
that can avoid adverse effects obtained from bacterial L-asparaginase, such as anaphylactoid reactions. Screening and selection
of the fungi and optimum concentration of the medium component are very important to determine the overall economic
feasibility of the production process. Therefore, the purpose of this study was to evaluate the important variables that influence
Lasparaginase activity by a filamentous fungus isolated from the Brazilian Savanna soil. Eleven independent variables were
considered to evaluate their effect on Lasparaginase activity by a filamentous fungus (DCFS10) in submerged fermentation.
The different variables were prepared in two concentration levels, (-1) low level and (+1) high level. L-asparaginase activity
was assayed by measuring the amount of aspartate hydroxamate produced from asparagine and hydroxylamine according
to Drainas et al. (1977). The results obtained from PBD showed a wide range of Lasparaginase activity, from 0.5 U/g ± 0.018
to 6.5 U/g ± 0.284. Studies are being conducted in order to purify L-asparaginase produced in this culture medium. This
study showed that the screening of culture medium variables using Plackett-Burman increased L-asparaginase activity of a
filamentous fungus isolated from the Brazilian Savanna soil as a potential novel anti-leukemic source from eukaryote cell.
perolamagalhaes@unb.br