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Journal of Biotechnology & Biomaterials | ISSN: 2155-952X | Volume: 8
3
rd
World Biotechnology Congress
December 03-04, 2018 Sao Paulo, Brazil
Statistical analysis for the production of secondary metabolites and its chemical characterization from
Streptomyces parvulus C5-5Y
Arockiam Jeyasundar Parimala Gnana Soundari
2
and
Vellingiri Manon Mani
1,2
1
Hindusthan College of Arts and Science, India
2
Bharathiar University, India
Introduction:
The needs for an increase in novel drugs urged to discover and develop new antibiotics with biopotential. The natural
products have been developed from medicinal plants and the recent research has mainly focused on the microbial sources for novel
antibiotics with bioactivities and this is economical in the state. In this investigation, we have developed a new bioactive compound
with maximum antioxidant and antimicrobial activities.
Methodology:
In order to increase the metabolite production as well as the organism’s growth we aimed to optimize the medium
with economical parameters and sources rapidly. The investigation followed the one factor at a time, Plackett-Burman design and Box
Behnken design to get the optimized medium for the enhanced metabolites production. Stat-Ease design expert software was used for
this investigation. On purification, the secondary metabolite which was exhibiting maximum activity at a minimum concentration
was taken for structural elucidation analysis through UV-VIS, FTIR, NMR and HRLC MS/MS analysis.
Results:
The bioactive compound was elucidated from
Streptomyces parvulus
. The maximum growth and pigment production were
evaluated with the standard formula and the production was higher in optimal pH, temperature, carbon and nitrogen sources. The
carbon sources are found to increase the growth of the organism, especially in starch. The mass production was obtained in the
optimized medium and the extracted pigments were subjected to HPLC analysis where the peak 4 was eluted and found to contain
bioactivity through antimicrobial assessment. The compound AP
4
was structurally elucidated with raw data, finally, the AP
4
was
4-MHA (4-Methyl 3-Hydroxy anthranilic acid) pentapeptide lactone and it represents the half actinomycin structure with antioxidant
properties. Further studies will be focused on two-dimensional NMR spectroscopy to confirm the structure and application of
pigment as pharma product in
in vivo
studies.
apgs_1987@yahoo.co.inJ Biotechnol Biomater 2018, Volume: 8
DOI: 10.4172/2155-952X-C7-107