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conference
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.com
Volume 7, Issue 6 (Suppl)
J Biotechnol Biomater, an open access journal
ISSN: 2155-952X
World Biotechnology 2017
December 04-05, 2017
2
nd
World Biotechnology Congress
December 04-05, 2017 | Sao Paulo, Brazil
Exploring detection methods for foodborne pathogens: Shiga toxin-producing
E. coli
as an example
F
oodborne pathogens are a growing concern for human health worldwide. The Center for Disease Control and Prevention
estimated that there are approximately 48 million new cases of foodborne illness each year, resulting in 128,000
hospitalizations and 3,000 deaths in the United States alone. To ensure food safety, demands for methods that are rapid and
reliable for detecting foodborne pathogens are increasing. As improvements in biotechnology, newmolecular methods, such as
DNA-based PCR and antibody-based immunoassays are being developed and improved continuously. These methods enable
faster and more sensitive detection of foodborne pathogens. However, challenges in food matrix effect, sample preparation,
detection and recovery of viable cells, and reducing test time still exist. This presentation reviews the evolution of detection
methods for foodborne pathogens using Shiga toxin
E. coli
as an example, discusses the deficiencies of currently available
methods in relation to the industry’s needs.
Biography
Xiaohua He is a Research Molecular Biologist at USDAARS, WRRC, Albany, California. She has received her PhD in Plant Pathology from University of California,
Riverside, and had Postdoctoral experience at Purdue and Cornell Universities. Her research focuses on development of molecular tools and technologies for
sensitive detection of zoonotic pathogens and toxins in food, environment and clinical samples; investigation of toxin synthesis and mechanisms of host cell injury
by toxins. She has received the 2015 USDA Federal Laboratory Consortium, Far West Region, Outstanding Technology Development Award for her contribution
to the development of novel monoclonal antibodies against a broad range of Shiga toxins. She has served as Academic Editor and Editorial Board Member of
leading journals. Her work has involved significant international collaborations, with applications to food safety and defense. She is an author/inventor of over 80
publications and patents, with 14 technologies licensed to industry.
Xiaohua.he@ars.usda.govXiaohua He
USDA, WRC, ARS, USA
Xiaohua He, J Biotechnol Biomater 2017, 7:6 (Suppl)
DOI: 10.4172/2155-952X-C1-084