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Volume 8 Journal of Traditional Medicine & Clinical Naturopathy
Page 47
June 20-21, 2019 Dubai, UAE
Joint Meeting on
&
2nd World Congress on Traditional & Complemenatry Medicine
2nd International Conference on Herbal & Traditional Medicine
Traditional Med Meet 2019 &
Herbal Traditional 2019
June 20-21, 2019
Bruno O Onyemegbulem et al., J Tradit Med Clin Natur 2019, Volume 8
Post-traumatic stress disorder and the toxicology of Cannabis sativa
Bruno O Onyemegbulem1,Onunekwu O Charles1, Yusuf N Omeh1and J C Aguiyi2
1University of Jos, Nigeria
2Michael Okpara University of Agriculture Umudike, Nigeria
Many youngmen, women and even the elderly are addicted toCannabis intake abuse despite its predictable
toxicological consequences. In this study we studied the toxic effects of oral administration of methanol
extract of Cannabis sativa seeds using total of fortymaleWistar rats. Animals randomizedwere into five groups
(n=8 rats) of approximately equal weight. Group-1 received 100 mg/kg of the of the extract, group-2 received
200 mg/kg of the extract, group-3 received 300 mg/kg dosage of the extract, group-4 received 2 ml of virgin
olive oil (vehicle control) and group-5 received distilled water (normal control) for 14 days. The rationale
behind the vehicle control group is to show that the vehicle did not have any extra cytological/histological
effect. The proximate properties show: Moisture content (6.69±0.14%), protein content (19.10±0.01%), ash
content (11.84±0.02%), fat (19.33±0.00%), crude fiber (18.87±0.00%), carbohydrate (43.04%), dry matter
(80.91±0.14%) and oil absorption capacity (1.87±0.00%). The anti-nutrients were as follows: alkaloids
(3.15±0.01 mg/100 g), flavonoids (2.82±0.01 mg/100 g), saponins (6.10±0.01%), tannins (2.14±0.01 mg/100
g), cyanogenic glycosides (0.10±0.01 mg/100 g) and phenols (0.27±0.01 mg/100 g). The mineral content of
the sample were potassium (13.73±0.01 mg/100 g), phosphorus (10.00±0.02 mg/100 g), sodium (17.53±0.01
mg/100 g), calcium (14.65±0.01 mg/100 g), magnesium (09.67±0.01 mg/100 g), zinc (2.00±0.01 mg/100 g)
and Iron (1.00±0.01 mg/100 g). Result for AST was significantly (p<0.05) higher in group-2 (57.00±13.00
IU/L) and group-3 (59.33±10.53 IU/L), when compared with the normal control group-5 (31.33±1.53
IU/L). Significantly (p<0.05) higher serum ALT was observed in treatment group-2 (50.00±12.52 IU/L) and
group-3 (56.33±10.21 IU/L). The results for kidney function, shows significantly (p<0.05) higher serum urea
concentration in group-3 (13.75±2.41 mg/dl) when compared with the normal control group (8.75±1.60
mg/dl). Serum creatinine concentration was significantly (p<0.05) higher in group-2 (2.25±1.18 mg/kg) and
group-3 (2.38±1.57 mg/kg) when compared with the normal control group (1.09±0.13 mg/kg). Significantly
(p<0.05) higher SOD values was obtained in group-3 (72.64±5.90 mg/kg) when compared with normal
control group (19.62±4.26 mg/kg). There was no significant (p<0.05) difference in total serum protein
concentration in all groups when compared with the normal control group. The histological result shows
that the oral administration of Cannabis sativa induced pronounced inflammation of the hepatic and renal
tissue in group-3 when compared with the normal control group. In conclusion, the study showed that oral-
administration of Cannabis sativa caused dose dependent hepatorenal toxicity.
Biography
Bruno O Onyemegbulem is the specialist of philosophy. He is associated with the African Center of Excellence in Phytomedicine Research
and Development University of Jos as a research fellow. He is the member of National Association of Mathematical Physics (NAMP),
Nigerian Mathematical Society (NMS) and Nigerian Society for Mathematical Biology (NSMB). He is the author of "A Mathematical Modelling
of the Effect of Treatment in the Control of Malaria in a Population with Infected Immigrants".