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Volume 5
Toxicology: Open Access
Toxicology Congress 2019
May 06-07, 2019
May 06-07, 2019 Tokyo, Japan
20
th
World Congress on
Toxicology and Pharmacology
Toxicol Open Access 2019, Volume 5
DOI: 10.4172/2476-2067-C1-009
Investigating brain tissue damage following respiratory contact with carbon nanotubes in rat using
isolated lung mitochondria
Fatemeh Samiei and Jalal Pourahmad Jaktaji
Iran University of Medical Sciences, Iran
Objective:
The main aim of this study is to investigate the lung toxicity following the respiratory contact with Multi-Wall
Carbon Nanotubes (MWCNTs) in male Wistar rats.
Method:
Rats were exposed to 5 mg/m3 MWCNT aerosol in different size and purity for 5 hours/day, 5 days/week for 2 weeks
in a whole-body exposure chamber. After two weeks exposure, the rats of all groups was necropsied the animals lungs were
removed. Then we separated the left and right lungs and mitochondria of them were isolated and parameters of mitochondrial
toxicity including mitochondrial succinate dehydrogenase (complex II) activity, generation of Reactive Oxygen Species (ROS),
Mitochondrial Membrane Potential (MMP) collapse, mitochondrial swelling and cytochrome c release were evaluated.
Result:
Our results demonstrated that MWCNTs with different characteristics, in size and purity, significantly (P<0.05)
decreased mitochondrial succinate dehydrogenase activity and mitochondrial ROS production. Induced mitochondrial
swelling, MMP collapse and cytochrome c release mitochondria and right lung had seen more damage.
Conclusion:
We concluded that MWCNTs with different characteristics, in size and purity because damage in varying degrees
on the mitochondrial respiratory chain and induce ROS mediated cytotoxicity by directly targeting mitochondria lung tissue.
It seems that the right lung has been more damaged due to the larger size of the left lung.
fh.samiei@yahoo.com