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Volume 8
Journal of Stem Cell Research & Therapy
ISSN: 2157-7633
Stem Cell Congress 2018
October 08-09, 2018
October 08-09, 2018 | Zurich, Switzerland
10
th
Annual Conference on
Stem Cell & Regenerative Medicine
In
situ
tissue engineering concept for enhanced bone defect regeneration– functionalization of
biomimetic scaffolds with an autologous growth factor mix from hypoxia-exposed hBMSC
Anastasia Gabrielyan
1
, Mandy Quade
1
, Anja Lode
1
, Michael Gelinsky
1
, Seemun Ray
2
, Jessica Grafe
2
, Volker Alt
2
and
Angela Rösen-Wolff
1
1
University Hospital Carl Gustav Carus, Germany
2
Justus Liebig University, Germany
T
he potential for self-regeneration of bone tissue is not sufficient to regain the original function in the case of extensive
lesions, osteoporosis, injury or tumor resection. Hence, the main goal of bone tissue engineering has been the generation
of biological substitutes which remodel into native tissue to replace affected bone.
In vivo
tissue regeneration depends on
migration of stem cells into injured areas, their differentiation into specific cell types and their interaction with other cells
that are necessary to generate new tissue. Therefore, optimized biomaterials are needed which allow survival and growth of
mesenchymal stem cells, a subset of bone marrow stromal cells (BMSCs), which can migrate and differentiate into osteoblasts
in bone tissue. Hypoxia-conditioned media (HCM) has a high chemo attractive capacity for BMSCs, as it harbors high
concentrations of growth factors which are important to stimulate angiogenesis and cell migration. It can be derived from
BMSCs but also from skin fibroblasts which can be easily obtained from patients in individualized therapy approaches. Scaffold
functionalization with a central growth factor depot enhances hBMSC infiltration as well as ingrowth of tubular endothelial
structures providing a strategy to stimulate
in situ
colonization with cells from the surrounding tissue. For
in vivo
testing, a 4
mm wedge shaped osteotomy of the distal metaphyseal area was generated in the femur of osteoporotic rats. Six weeks after
implantation of mineralized collagen scaffolds loaded withHCM, bone defect healing was characterized histomorphometrically
revealing an enhancing effect on vascularization and new bone formation. In our work, we demonstrated that allogenous
growth factor mix derived fromHCM is suitable to attract cells with regenerative potential, induces vascularization
in vitro
and
has been shown to enhance bone defect healing
in vivo
.
Recent Publications
1. Quade M,
et al.,
(2018) Strontium-modification of porous scaffolds from mineralized collagen for potential use in bone
defect therapy. Materials Science & Engineering C Materials Science & Engineering 84:159-167.
2. Gabrielyan A,
et al.,
(2017) Metabolically conditioned media derived from bone marrow stromal cells or human skin
fibroblasts act as effective chemo attractants for mesenchymal stem cells. Stem Cell Research &Therapy 8(1):212.
3. Quade M,
et al.,
(2017) Central growth factor loaded depots in bone tissue engineering scaffolds for enhanced cell
attraction. Tissue Engineering Part A 23(15-16):762-772.
4. Gabrielyan A,
et al.,
(2014) Hypoxia-conditioned media allows species-specific attraction of bone marrow stromal cells
without need for recombinant proteins. BMC Veterinary Research 10(1):56.
5. Alt V,
et al.,
(2013) A newmetaphyseal bone defect model in osteoporotic rats to study biomaterials for the enhancement
of bone healing in osteoporotic fractures. Acta Biomaterialia 9(6):7035–7042.
Biography
Anastasia Gabrielyan studied Biology at TU Dresden and is currently a PhD student at the University Hospital Carl Gustav Carus, Dresden. Her research has been
published in reputed journals.
anastasia.gabrielyan@uniklinikum-dresden.deAnastasia Gabrielyan et al., J Stem Cell Res Ther 2018, Volume 8
DOI: 10.4172/2157-7633-C4-041