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Volume 8
J Community Med Health Educ, an open access journal
ISSN: 2161-0711
Public Health 2018
February 26-28, 2018
PUBLIC HEALTH AND NUTRITION
3
rd
World Congress on
February 26-28, 2018 London, UK
J Community Med Health Educ 2018, Vol 8
DOI: 10.4172/2161-0711-C1-033
GRAPEFRUITALCOHOLICSEEDEXTRACTEFFECTONCANDIDAALBICANSRESISTANT
TO FLUCONAZOLE AND CLOTRIMAZOLE
Fatemeh Fallah
a
, Gita Eslami
a
and
Fattaneh Fallah
a
a
Shahid Beheshtin University, Iran
Grapefruit Seed Extract (GSE) has been shown to possess antibacterial, antiviral, antifungal and antiparasite properties. It
contains large quantities of polyphenolic compounds, such as catechins, epicatechin, epocatechin-3-O-gallate, dimeric,
trimeric and tetrameric procyanidins. These beneficial actions of GSE have partly been attributed to the antioxidative activity
of citrus flavonoids, such as naringenin In the current study, the efficacy of GSE on Candidia albicans resistant to Clotrimazole
and fluconazole, collected from vaginitis samples was investigated. Vaginal secretions of 100 women with vaginitis referred
to health care centers were studied. Tests for microbial diagnosis and common drug resistance to microbial samples were
performed. MIC method was compared to Disk diffusion. Also samples were exposed to Grapefruit seed hydroextract in
various concentrations supplied with decoction method and grapefruit seed alcohol extract by MIC method. There were 100
samples collected from women aged 20 - 50 years vaginitis was more prevalent in age range 25 - 30 years. Of all samples 40
were candida positive and 60 had bacterial infection. Candida samples mostly resisted to Clotrimazole, of all cases 30% were
resistant to clotrimazole and 17.5% to fluconazol. Samples were exposed to hydroextract, no antifungal effect was obsereved,
and also they were exposed to Alcoholic extract, prepared with maceration method, in various concentrations with MIC
method. Alcoholic GSE with 10 and 20% had effect on 75% of candida samples resistant to Clotrimazole and fluconazole. Our
results suggest that alcoholic GSE could inhibit Candidia albicans growth significantly (P < 0.05); GSE might be used as an
effective and safe product for fungal vaginitis.