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conferenceseries
.com
June 26-27, 2017 San Diego, USA
13
th
International conference on
Pathology and Molecular Diagnosis
Volume 7, Issue 2 (Suppl)
J Clin Exp Pathol, an open access journal
ISSN:2161-0681
Pathology and Molecular Diagnosis 2017
June 26-27, 2017
Investigation for polymorphisms of
caspase 3
and
caspase 9
gene and enzyme levels in leukemia
patients
Melek Meydanogulları, Nazan Eras, Naci Tiftik, Anil Tombak and Etem Akbas
Mersin University, Turkey
F
orunderstandingof leukemia and its treatmentwithearlydiagnosis, themolecular changes that occurduring thedevelopment
of leukemia should be investigated. Apoptosis is central to the development and homeostasis of the hematopoietic system.
Previous studies have reported that leukemia cells invariably have abnormalities in one or more apoptotic pathways.The current
study investigated the relationship between polymorphisms of
caspase 3
G>T rs4647601 and
caspase 9
A>G rs4645978 and
leukemia. Besides that, we aimed to determine
caspase 3
and
caspase 9
enzyme levels possible effects on the risk of developing
leukemia. The case group consisted of 100 patients (mean age: 56±03) who had been newly diagnosed with leukemia at the
Department of Hematology, Mersin University Faculty of Medicine, Turkey. The control group comprised of 100 healthy
properly age and sex matched individuals (mean age: 54±15) with a no history of leukemia. The genotypes were detected by
using Real-Time PCR. We measured enzyme levels of
caspase 3
and 9 in serum, which were obtained from blood samples.
No significant association was observed between
caspase 3
G>T rs4647601 and
caspase 9
A>G rs4645978 polymorphisms and
leukemia. We found that median levels of
caspase 3
and 9 were higher in leukemias than in normal blood cells (P<0.001). This
is the first study reporting the detailed distribution of alleles and genotypes of
caspase 3
and
caspase 9
in leukemia patients in
Turkish population. Taken together, we conclude that
caspase 3
and
caspase 9
levels may be useful for the early diagnosis of
leukemia.
Biography
Nazan Eras, after graduation from Dicle University Faculty of Medicine, worked as a General Practitioner. She completed her MSc in 2006 and PhD in 2012 at
Mersin University Faculty of Medicine, Department of Molecular Biology and Genetics. She has been serving as Peer-reviewer in journals. She still continues to
work as a Scientist and an Assistant Professor at Mersin University Medical Faculty, Department of Medical Genetics. Her research interests include Clinical Cancer
Genetics, Human Molecular Genetics and Oxidative Stress.
nazaneras@gmail.comMelek Meydanogulları et al., J Clin Exp Pathol 2017, 7:2 (Suppl)
DOI: 10.4172/2161-0681-C1-034