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conferenceseries.com
June 26-27, 2017 San Diego, USA
13th International conference on
Pathology and Molecular Diagnosis
Volume 7, Issue 2 (Suppl) J Clin Exp Pathol, an open access journal
ISSN:2161-0681
Pathology and Molecular Diagnosis 2017
June 26-27, 2017
Investigation of relationship between -1195 A>G polymorphism of COX-2 gene and mRNA levels
of COX-2 gene in peripheral blood monocyte in colorectal cancer patients
Hicran Şenli, Leyla Bahar, Nazan Eras, Tahsin Çolak, Mehmet Ozgur Turkmenoglu, Seval Kul and Etem Akbas
Mersin University, Turkey
C olorectal cancer (CRC) arises from the colorectal epithelium as a result of the accumulation of genetic alterations in
defined oncogenes and tumor suppressor genes. The molecular changes occurring during the development of the tumor
must be investigated in order to understand the carcinogenesis. The cyclooxygenase (COX) isoenzymes, COX-1 and COX-2,
catalyze the formation of prostaglandins, thromboxane, and levuloglandins. COX-2 is induced by inflammatory and mitogenic
stimulants and prevails on tumor carcinogenesis by increasing the prostaglandin synthesis in inflammatory and neoplastic
tissues. The aim of this study was to investigate the association the COX-2 gene -1195 A>G polymorphism and CRC risk.
We also investigated the relationship between the COX-2 gene mRNA levels in peripheral blood monocytes and -1195 A>G
polymorphism in CRC. Ninety individuals with CRC and 106 healthy individuals are included in our study. The genotypes are
determined by using PCR-RFLP. RNA of individuals with CRC is isolated and RT-PCR is applied. Genotype distribution and
allelic frequencies for -1195 A>G polymorphism of COX-2 gene weren’t significantly different between patients and controls.
COX-2 gene mRNA levels and genotype distributions of this polymorphism has no difference between CRC patients and
controls. While one of the other factors of developing CRC; the advanced age and male gender increases the risk of developing
CRC, BMI, smoking and alcohol intake have no effect on risk of developing CRC. Our study is the first study to investigate the
relation between -1195 A>G polymorphism and mRNA levels of COX-2 gene in CRC in Turkish population.
Biography
Leyla Bahar, after graduating from Cukurova University Faculty of Medicine, worked as a Medical Practitioner in Mersin, until 2002. In the Department of Histology-
Embryology, she completed PhD in Mersin University Faculty of Medicine in 2008. She has published more than 20 papers and announcement in journals and
has been serving as a Consultant Editor and Editorial Board Member of reputable journals. She still continues to work as a Scientist and an Assistant Professor at
Mersin University, who is working on many issues and as peer-reviewer in journals.
Hicran Şenli et al., J Clin Exp Pathol 2017, 7:2 (Suppl)
DOI: 10.4172/2161-0681-C1-034