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Page 53

Parkinsons 2016

December 05-07, 2016

Volume 6 Issue 6(Suppl)

J Alzheimers Dis Parkinsonism

ISSN: 2161-0460 JADP, an open access journal

conferenceseries

.com

December 05-07, 2016 Phoenix, USA

2

nd

International Conference on

Parkinson’s Disease & Movement Disorders

Alan Wu et al., J Alzheimers Dis Parkinsonism 2016, 6:6(Suppl)

http://dx.doi.org/10.4172/2161-0460.C1.025

Grape skin powder mediates mitochondria function by autophagy activation and exhibits potential

protective benefit in a

Drosophila

Parkinson’s Disease Model

Alan Wu

1,2

, Zhihao Wu

1

, Andy Sigears

2

and

Bingwei Lu

1

1

The Department of Pathology, School of Medicine, Stanford University, Palo Alto, CA

2

Crystal Springs Uplands School, Hillsborough, CA

R

ecent studies suggest that moderate red wine consumption may confer several health benefits: longer lifespan, protection

against heart diseases, certain cancers and age-related neurological diseases. These health benefits are believed to come

from a compound called resveratrol. Here we investigate the potential effect of grape skin from pure merlot on Parkinson’s

disease by incorporating grape skin powder into the daily food intake of

Drosophila melanogaster

with

PINK1

loss-of-function.

The benefits of consuming this grape skin powder have featured not only the improvement of indirect flight muscle functions,

as shown in the rescue of abnormal wing posture in

PINK1

mutant flies, but also prolonged the lifespan. The effect on WT

flies’ life span is not significant. Mitochondrial dysfunction is linked to the pathogenesis of Parkinson’s disease, in particular,

PINK1

has been suggested to interact with mitochondrial fusion/fission machinery and the autophagy pathway. To underscore

the beneficial qualities of the grape skin, we further showed that consumption of the grape skin powder demonstrated a rescue

of mitochondria aggregation phenotype in the muscle of

PINK1

mutants. Moreover; results from western blots exhibited

significantly elevated levels of LC3-II in the muscles of grape powder fed flies, indicating increased mitochondria autophagy.

This effect is more obvious in flies fed with grape skin than the pure resveratrol compound. In addition, mutant flies appeared

to be more sensitive than wild type flies. Our study suggested grape skin powder can induce autophagy activation, mediate the

mitochondria function, and has potential protective benefit in a Parkinson's disease model.

Biography

Alan Wu is an intern student working in Dr. Bingwei Lu’s lab in Stanford University School of Medicine. He is currently a Junior in the Crystal Springs Uplands High

School. This study is done with the instruction and guidance from his mentor, Dr. Zhihao Wu, who is a post-doc in Dr. Lu’s lab.

awu@crystal.csus.org