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conferenceseries
.com
Volume 8
Journal of Obesity &Weight Loss Therapy
Obesity Summit 2018
July 10-11, 2018
July 10-11, 2018 Bangkok, Thailand
19
th
World Obesity Congress
Recuperative effect of nesfatin-1 in the spermatogenesis and steroidogenesis in the diabetes mice
Ashutosh Ranjan and Amitabh Krishna
Banaras Hindu University, India
Introduction & Aim:
Recent investigation has reinforced and validated the nesfatin-1 as an adipokine principally involved
in whole body energy homeostasis. It imparts several physiological roles including puberty onset, sleep, stress regulation,
behavior response, reproduction, etc. This study was aimed to evaluate the effect of nesfatin-1 in the regulation of testicular
physiology in the diabetic mice.
Method:
The diabetic mice were treated intraperitoneally for 14 days (SD) with 1.25 nM/gbw nesfatin-1. The treatment
produced significant changes in the spermatogenesis and steroidogenesis activity in the diabetic mice.
Result:
Nesfatin-1 treated diabetic mice showed increased proliferation of germ cells as indicated by increased accumulation
of spermatocytes and round spermatid in the seminiferous tubule. Nesfatin-1 treatment increases the testicular expression
of Proliferating Cell Nuclear Antigen (PCNA) and B-Cell Lymphoma-2 (BCL-2) expression compared to diabetic control
group mice, which further support the importance of nesfatin-1 in germ cell proliferation, their survival and spermatogenesis.
The diabetic mice treated with nesfatin-1 showed significant increase in testosterone synthesis compared to diabetic control
mice due to stimulatory effect of nesfatin-1 on testicular 3 beta HSD activity and increased expression of Steroidogenic Acute
Regulatory protein (StAR) and Luteinizing Hormone (LH-receptor) proteins.
Conclusion:
In addition, nesfatin-1 treatment also showed increased glucose transport by increasing the expression of glucose
transporter (GLUT-8) and Insulin Receptor (IR) proteins in the testis. This study further explored the increased production
of testosterone may be mediated via increased production of nitric oxide. Altogether, the study suggests the stimulatory role
of nesfatin-1 in the regulation of testicular steroidogenesis and spermatogenesis, including testicular metabolism in diabetic
mice.
ashutoshranjan.bhu@gmail.comJ Obes Weight Loss Ther 2018, Volume 8
DOI: 10.4172/2165-7904-C4-067