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.com
Volume 7, Issue 6 (Suppl)
J Obes Weight Loss Ther
ISSN: 2165-7904 JOWT, an open access journal
Obesity Meeting 2017
October 23-24, 2017
October 23-24, 2017 Dubai, UAE
14
th
Global Obesity Meeting
RFRP-3 acts as a mediator between obesity and impaired testicular function
Shabana Anjum
1
, Amitabh Krishna
1
and Kazuyoshi Tsutsui
2
1
Banaras Hindu University, India
2
Waseda University, Japan
T
he aim of the present study was to evaluate the roles of RFamide related peptide-3 (RFRP-3) a novel hypothalamic
neuropeptide, as an endocrine link between increasing adiposity and impaired testicular function in mice. To achieve
this, the effect of RFRP-3 on changes in nutrients uptake and hormonal synthesis/action in the adipose tissue and testis was
investigated simultaneously by
in vivo
study and separately by
in vitro
study. Mice were treated
in vivo
with different doses of
RFRP-3 for 8 days. In the
in vitro
study, adipose tissue and testes of mice were cultured with different doses of RFRP-3 with
or without insulin or LH for 24 hours at 37
o
C. The RFRP-3 treatment
in vivo
showed increased food intake, up-regulation of
glucose transporter 4 (GLUT4) and increased uptake of triglycerides in the adipose tissue. These changes may be responsible
for increased accumulation of fat into white adipose tissue, resulted in increase in the body mass. On the contrary to the
adipose tissue, treatment with RFRP-3 both
in vivo
and
in vitro
showed decreased uptake of glucose by down-regulation of
glucose transporter 8 (GLUT8) expressions in the testes, which in turn resulted in the decreased synthesis of testosterone.
The RFRP-3 treatment
in vivo
also showed the decreased expression of insulin receptor protein in the testis which may also
be responsible for the decreased testicular activity in the mice. These findings thus suggest that RFRP-3 increases the uptake
of glucose and triglycerides in the adipose tissue resulting in increased accumulation of fat, whereas simultaneously in the
testis RFRP-3 suppressed the GLUT8 mediated glucose uptake, which in turn may be responsible for decreased testosterone
synthesis. This study thus demonstrates RFRP-3 as mediator of increasing adiposity and impaired testicular function in mice
and may be used as therapeutic agent for male infertility.
Biography
Shabana Anjum has completed her PhD in 2015 from Banaras Hindu University, India. She has published 5 papers and book chapters in reputed international
journals. She has presented many papers in national and international conferences.
anjum6repro@gmail.comShabana Anjum et al., J Obes Weight Loss Ther 2017, 7:6 (Suppl)
DOI: 10.4172/2165-7904-C1-051