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conferenceseries.com
February 20-21, 2017 Berlin, Germany
9th International Congress on
Nutrition & Health
Volume 7 Issue 1 (Suppl) J Nutr Food Sci
ISSN:2155-9600 JNFS, an open access journal Nutrition & Health 2017
February 20-21, 2017 Berlin, Germany
Abdulkarim Dakah, J Nutr Food Sci 2017, 7:1 (Suppl)
Evaluation of antioxidant activity of wild medicinal plant Ziziphora tenuior Land using plant tissue culture
to increase its activity
Abdulkarim Dakah
International University for Sciences and Technology, Syria
M edicinal plants played an important role in the treatment of diseases and health disorders for thousands of years and are still
important in traditional medicine systems around the world. Ziziphora tenuior L. (Lamiaceae) is an aromatic herb used for its
medicinal values against fungi, bacteria. Z. tenuior has been used to treat fever, dysentery, diarrhea, gut inflammation, cough, bladder
stones, and painful menstruation. For increased human needs of medicines, plant tissue culture is used widely for micro-propagation
of medicinal plants to produce enough amounts of drugs and secondary metabolites, using this technology, the natural products can
be provided at any time of the year without waiting for the suitable season to collect the plant. This work aims to develop a reliable
protocol for the in vitro propagation of Z. tenuior , and increase active substances to compare the antioxidant activity between in vitro
propagated and wild plants. The explants were sterilized and cultured on MS medium containing different concentrations of growth
regulators naphthalene acetic acid (NAA) or indole-3-butyric acid (IBA) with 0.5 mg/L of kinetin (Kin) callus formation was 70.2%
after 45 days of incubation in dark on medium supplemented with 1.5 mg/L of NAA. After one month of callus culture on medium
supplemented with 2 mg/L BA the shoot number was 5.12 and for the multiplication stage. The shoot number was 4.21 and length
was 6.17 cm on medium supplemented with 1 mg/L Kin +0.1 mg/L NAA. DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent was used to
test the antioxidant activity. The aqueous and methanol extracts of in vitro plants which were treated with 1.5 and 1 mg/L of kin plus
0.1 mg/L of NAA showed a strong DPPH scavenging activity where IC50 was 0.307 and 0.369 mg/ml, respectively, while the IC50 of
aqueous and methanol extracts of wild plants was 0.516 and 9.229 mg/ml, respectively.
Biography
Abdulkarim Dakah is a Doctor at Damascus University, he has done his Doctorate in Plant Biology, worked at the International University for Sciences and
Technology, Kalamoon University and Damascus University. He has a lot of Laboratory skills, like: test of bioactivity of plants, plant tissue culture and molecular
characterization. He has published many articles in international journals. He has done his work on Bio-pesticidal Proteins: from isolation to molecular
characterization and applications and has been certified by the Atomic Energy Commission of Syria in cooperation with International Centre for Genetic Engineering
and Biotechnology (ICGEB). He has reviewed a lot of manuscripts for international journals.