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Volume 6, Issue 3 (Suppl) J Neonatal Biol, an open access journal
ISSN:2167-0897 Neonatology 2017
December 04-06, 2017
December 04-06, 2017 | Madrid, Spain
20th International Conference on
NEONATOLOGY AND PERINATOLOGY
Endothelial microvesicles in patients on extracorporeal membrane oxygenation: Potential markers
of systemic inflammation and organ dysfunction?
K Žilinská1, V Vítková1,2, M Pánek3,2, P Janec3,2, V Vobruba4, J Živný2 and J Janota 1,2
1Thomayer Hospital Prague, Czech Republic
2Charles University, Czech Republic
3Masaryk Hospital Usti nad Labem, Czech Republic
4General University Hospital in Prague, Czech Republic
Introduction&Aim: Inflammatory response influences mortality andmorbidity. Processes leading to a systemic inflammation
are associated with endothelial injury. Endothelial cells release microvesicles and several other biomarkers which can be used
as diagnostic tools for detection and treatment of inflammatory response. There is limited information regarding endothelial
dysfunction in newborns. Aim of our study was to explore microvesicles in critically ill newborns.
Methods: Microvesicles were measured in newborns on extracorporeal membrane oxygenation (ECMO) and compared
to samples of healthy term newborns. The total microvesicle count and number of surface antigen-specific microvesicles
was determined by flow cytometry (BD FACS CantoII). The plasma concentration of cell-derived microvesicles (MV) was
measured using annexin V labeling of the microvesicles and the endothelial origin of microvesicles were determined using
lineage specific antigen labelling of endothelial cell/microvesicle markers (CD105, CD31, CD309, MadCAM).
Results: 13 newborns on extracorporeal membrane oxygenation (ECMO group) and 13 healthy term newborns (Term
group) were included. There were no significant differences in gestational age, birthweight and gender between groups. The
concentration of markers shows Table1.
Conclusion: Soluble markers of inflammation and concentration of microvesicles were significantly increased in ECMO group.
We did not detect significant differences in the concentration of endothelial specific microvesicles between the groups, with
the exception of mucosal endotheliummarker MadCAM. The understanding of endothelial cell response during inflammation
may help to find biomarkers of early detection of neonatal diseases.
Biography
V Vitkova graduated from Faculty of Medicine Charles University, Prague in 2012 and started her PhD studies in 2016 at Charles University, Department of
Pathological Physiology. Currently, she works as a Pediatrician/Neonatologist in Department of Neonatology, Thomayer Hospital, Prague, Czech Republic
K Žilinská et al., J Neonatal Biol 2017, 6:3(Suppl)
DOI: 10.4172/2167-0897-C1-006