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Volume 6, Issue 3 (Suppl)
J Neonatal Biol, an open access journal
ISSN:2167-0897
Neonatology 2017
December 04-06, 2017
December 04-06, 2017 | Madrid, Spain
20
th
International Conference on
NEONATOLOGY AND PERINATOLOGY
The investigation on the protective role of regulatory T cells in LPS induced fetal liver damage in late
pregnant mice
Muhammad Siddiq and Li Liu
Xi’an Jiaotong University College of Medicine, China
T
o evaluate the role of regulatory T cells (Tregs) on the liver inflammatory response in a lipopoly- saccharide (LPS)-induced
preterm birth mouse model. The LPS-induced preterm birth mouse model was established. Before LPS treatment, Tregs
were insulated from pregnant mice and inoculated into different pregnant mice. The expression of Heme oxygenase-1 (HO-1),
fork head family transcription factor (Foxp3) and interleukin-6 (IL-6) in liver, were examined by real-time reverse transcription
polymerase chain reaction and western blotting. The mRNA and protein expression levels of, HO-1 and Foxp3 in liver from
LPS-treated mice was considerably reduced equated with the controls, while the adoptive transfer of Tregs expressively
rescinded the changes in the expression of the above said elements after LPS treatment. Fascinatingly, the expression of IL-6 in
the liver was meaningfully elevated after LPS treatment, and the adoptive transfer of Tregs obstructed this effect. The preterm
birth was remarkably persuaded after maternal LPS exposure, and affected the expression of Foxp3, HO-1and IL-6 in liver
tissue. Furthermore, the adoptive transfer of Tregs absolutely abolished the changes in the expression of the above factors after
LPS treatment. However, further study is needed to understand the mechanism of Tregs to prevent the liver inflammation in
preterm birth in human.
siddiq363@yahoo.comJ Neonatal Biol 2017, 6:3(Suppl)
DOI: 10.4172/2167-0897-C1-006