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conferenceseries.com
Volume 7, Issue 6 (Suppl) J Nanomed Nanotechnol
ISSN: 2157-7439 JNMNT, an open access journal Nanotek 2016
December 05-07, 2016
December 05-07, 2016 Phoenix, USA
13th International Conference on
Nanotek & Expo
Sara Malih et al., J Nanomed Nanotechnol 2016, 7:6 (Suppl)
Effects of silver nanoparticle on oxidative stress biomarkers in liver mitochondria male rat: In vitro study
Sara Malih, Mona Pourjafar, Rahmat Seyedabadi, Akram Ranjbar
Hamadan University of Medical Sciences, Iran
Nanotechnology and nanoparticles are increasingly recognized for their potential applications in aerospace engineering,
nanoelectronics, environmental remediation, medical healthcare and consumer products. As some nanoparticles can cross the
cell membranes particularly mitochondria and mitochondria has a vital role in the regulation of cellular energy through aerobic
pathway and electrolyte homeostasis, therefore the defects in mitochondrial function indued by nanoparticles can have severe effects
on cellular function. The purpose of this study was to investigate the effect of AgNP on the oxidative stress biomarkers in rat liver
mitochondia during different time intervals. In this study, the isolated mitochondria from the liver of male Wistar rats (180-250 g)
were used. Isolated mitochondria were treated with different doses (5, 1, 50, 100, 250 mmol/ml) of 20 nm diameter AgNP respectively
for 24, 48 and 72 hours. After treatment, oxidative stress biomarkers such as Total Antioxidant Capacity (TAC), Lipid Peroxidation
(LPO), Total Thiol Groups (TTG) and Catalase Activity (CAT) were determined in isolated rat liver mitochondria. The results
confirm oxidant/antioxidant effects of AgNP in various time. Results of this study showed that AgNP has paradox properties in liver
mitochondial sample, while mitochondria is a source of reactive oxygen species (ROS).
Biography
Sara Malih has completed her MSc in Medical Biotechnology from Hamadan University of Medical Sciences. She has published more than three papers in reputed
journals. She has two more research articles under review. She is Reviewer of Tumor Biology-Springer and has participated in more than three international
congresses.