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conferenceseries.com
Volume 7, Issue 6 (Suppl) J Nanomed Nanotechnol
ISSN: 2157-7439 JNMNT, an open access journal Nanotek 2016
December 05-07, 2016
December 05-07, 2016 Phoenix, USA
13th International Conference on
Nanotek & Expo
Radik Khayrullin et al., J Nanomed Nanotechnol 2016, 7:6 (Suppl)
The study of the cell membrane of blood lymphocytes in patients with diabetes mellitus using atomic force
microscopy
Radik Khayrullin, Olga Stolbovskaya and Rinat Bakhtiyarov
Ulyanovsk State University, Russia
Atomic Force Microscopy (AFM) is a nanotechnological study method of cell membranes and makes it possible to study the
structural changes in the cell membranes of living human blood lymphocytes. With using AFM, we studied Young's modulus,
adhesion strength and roughness of the cytoplasmic membrane of living blood lymphocytes in patients with diabetes. Peripheral
blood lymphocytes of patients with insulin-dependent and non-insulin dependent diabetes mellitus and healthy donors was analyzed
using a scanning probe microscope. The scanning of the cell surface membrane of blood lymphocytes were performed using silicon
probes with a hardness of 0.06 N/m, and with the radius of curvature of 10 nm. The results of studies have shown that diabetes is a
significant increase of Young's modulus of membrane of blood lymphocyte as compared with that in blood lymphocytes in healthy
persons. Also significant differences are in the adhesion properties and surface topography of lymphocytes in different types of
diabetes. In patients with insulin-dependent diabetes mellitus, the adhesion force and values of roughness of cells is higher than that in
patients with non-insulin dependent diabetes mellitus. Biophysical indicators reflect changes in the molecular structural organization
of membrane lymphocytes occurring in diabetes, however, it remains unknown how these changes determine the biological activity
of lymphocytes. The present study demonstrates and opens possibilities for the study of structural relationships of proteins and lipids
of native cell membranes of lymphocytes, understanding the role of lymphocytes in the development of immunological disorders,
occurring in this disease.
Biography
Radik Khayrullin has completed his PhD from Bashkir State Medical University (Russia) and Post-doctoral studies from First Moscow State Medical University. He
is a Professor, and Head of Human Anatomy Department of Ulyanovsk State University. He has published more than 80 papers in reputed journals and has been
serving as an Editorial Board Member of repute.