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Clinical Microbiology: Open Access | ISSN: 2327-5073 | Volume: 7
Microbiology: Education, R&D and Market
7th Annual Summit on
September 28-29, 2018 | San Antonio, USA
Interaction of the human respiratory Syncytial virus matrix protein with cellular adaptor protein
complex 3 plays a critical role in trafficking
Manoj Pastey
Oregon State University, USA
Human Respiratory Syncytial Virus (HRSV) is a leading cause of bronchopneumonia in infants and the elderly. To
date, knowledge of viral and host protein interactions within HRSV is limited and are critical areas of research. Here,
we show that HRSV Matrix (M) protein interacts with the cellular adaptor protein complex 3 specifically via its medium
subunit (AP-3Mu3A). This novel protein-protein interaction was first detected via yeast-two hybrid screen and was further
confirmed in a mammalian system by immunofluorescence colocalization and co-immunoprecipitation. This novel
interaction is further substantiated by the presence of a known tyrosine-based adaptor protein MU subunit sorting signal
sequence, YXXФ: where Ф is a bulky hydrophobic residue, which is conserved across the related RSV M proteins. Analysis
of point-mutated HRSV M derivatives indicated that AP-3Mu3A- mediated trafficking is contingent on the presence of the
tyrosine residue within the YXXL sorting sequence at amino acids 197-200 of the M protein. AP-3Mu3A is up regulated
at 24 hours post-infection in infected cells versus mock-infected HEp2 cells. Together, our data suggests that the AP-3
complex plays a critical role in the trafficking of HRSV proteins specifically matrix in epithelial cells. The results of this
study add new insights and targets that may lead to the development of potential antivirals and attenuating mutations
suitable for candidate vaccines in the future.
Biography
Manoj Pastey is a Diplomate ACVM and presently working as an Associate Professor and is the head of Molecular Diagnostic Laboratory, Department of Biomedical
Sciences in College of Veterinary Medicine, Oregon State University, Corvallis, USA.
Manoj Pastey, Clin Microbiol 2018, Volume: 7
DOI: 10.4172/2327-5073-C3-040