microbiology-and-mycotoxins-2017_scientifictracks-abstracts

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Volume 6, Issue 1 (Suppl)

Clin Microbiol

ISSN: 2327-5073 CMO, an open access journal

Microbiology & Mycotoxins 2017

February 27-28, 2017

February 27-28, 2017 Amsterdam, Netherlands

Euro Global Summit on

Clinical Microbiology and Mycotoxins

Simultaneous identification of

Mycoplasma gallisepticum

and

Mycoplasma synoviae

by duplex PCR assay

Golbarg Malekhoseini, Seyed Ali Pourbakhsh, Ali Reza Homayounimehr, Mohammad Reza zolfeghari, Abass Ashtari

and

Ali Reza Abtin

Islamic Azad University of Arak, Iran

ycoplasma gallisepticum

(M.G) and

Mycoplasma synoviae

(M.S) have been recognized as common respiratory pathogens

especially in chickens causing lots of economic losses in poultry industries. The aim of this study was to develop and validate

duplex polymerase chain reaction (PCR) for simultaneous detection of MG & MS. A total of 50 samples from tracheas, lungs and air

sacs were taken from commercial broiler chicken farms in Iran. The samples were cultured in PPLO broth supplemented for M.S and

M.G isolation and bacteria DNA were extracted by phenol/chloroform extraction method. The conserved region of

16S rRNA

gene

was applied for the detection of Mycoplasma genus in 163bp fragment and MG in 183 bp fragment and

vlhA

gene was also employed

for detection of MS in 350 bp fragment. Hence, duplex PCR amplified the conserved region of 16S

rRNA

and

vlhA

genes which were

then applied for detection of MG & MS. 20 samples in Mycoplasma genus, and 7 samples in MG & MS were positive in the single

PCR; whereas in 3 samples, MG & MS were simultaneously positive in the duplex PCR method. The results showed that duplex PCR

was successful to simultaneous identification of MG &MS and suggested that duplex PCR is more rapid and inexpensive method than

the single PCR for detection of MG & MS.

Biography

Golbarg Malekhoseini has finished her MSc in 2011 from Qom University. Currently, she is Assistant Professor at Islamic Azad University of Arak. Also, she is

working as a Manager of Quality Control in ice factory of Arak. His group simultaneously identified

Mycoplasma gallisepticum

and Mycoplasma synovia by duplex

PCR assay at Vaccine Institute of Karaj.

g1_malekhoseini@yahoo.com

Golbarg Malekhoseini et al., Clin Microbiol 2017, 6:1(Suppl)

http://dx.doi.org/10.4172/2327-5073.C1.027