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Volume 9
Journal of Analytical & Bioanalytical Techniques
Mass Spectra & Analytika 2018
September 19-20, 2018
September 19-20, 2018 Singapore
World Congress on
Mass Spectrometry and Analytical Techniques
Maria Ramos Payan et al., J Anal Bioanal Tech 2018, Volume 9
DOI: 10.4172/2155-9872-C2-030
A comprehensive study of a new versatile device based liquid phase micro extraction for stopped-flow
and double-flow conditions in a single step
Maria Ramos Payan and Garcia Gonzalez
University of Seville, Spain
A
new geometry for a versatile microfluidic-chip device based liquid phase micro extraction was developed to enhance
the preconcentration in microfluidic chips and to enable double-flow and stopped-flow working modes. The microchip
device was combined with a HPLC procedure for the simultaneous determination of two different families as model analytes,
which were parabens and Non-Steroidal Anti-Inflammatories (NSAIDs). The new miniaturized microchip proposed in this
work allows not only the possibility of working in double-flow conditions, but also under stagnant conditions (stopped-flow)
(SF-µLPME). The sample (pH 1.5) was delivered to the SF-µLPME at 20 µL min
-1
while keeping the acceptor phase (pH
11.75) under stagnant conditions for 20 minutes. The highest enrichment factors (between 16 and 47) were obtained under
stopped-flow conditions at 20 µL min
-1
(sample flow rate) after 20 minutes of extraction whereas the extraction efficiencies
were within the range of 27-81% for all compounds. The procedure provided very low detection limits between 0.7 and 8.5
µgL
-1
with a sample volume consumption of 400 µL. Parabens and NSAIDs have successfully been extracted from urine
samples with excellent clean up and recoveries over 90% for all compounds. In parallel, the new device was also tested under
double-flow conditions, obtaining good but lower enrichment factors (between 9 and 20) and higher extraction efficiencies
(between 45 and 95) after 7 minutes of extraction, consuming a volume sample of 140 µL. The versatile device offered very high
extraction efficiencies and good enrichment factor for double-flow and stopped-flow conditions, respectively. In addition, this
new miniaturized SF-µLPME device significantly reduces the costs compared to the existing analytical techniques for sample
preparation since this microchip requires few microliters of sample and reagents and it is reusable.
Biography
Maria Ramos Payan has pursued her PhD from University of Seville, Spain and Postdoctoral studies from University of Copenhagen, Denmark, University of North
Carolina, USA and Microelectronic National Center of Barcelona, Spain. She is the Leader of the microfluidic research line. She has published more than 30 papers
in reputed journals and has been serving as an Editorial Board Member of repute.
ramospayan@us.es