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Volume 9

Journal of Analytical & Bioanalytical Techniques

Mass Spectra & Analytika 2018

September 19-20, 2018

September 19-20, 2018 Singapore

World Congress on

Mass Spectrometry and Analytical Techniques

Yingying Wen, J Anal Bioanal Tech 2018, Volume 9

DOI: 10.4172/2155-9872-C2-030

Recent advances in sample preparation methods

Yingying Wen

Hainan Medical University, China

S

ample preparation is a crucial bottleneck in the whole analytical process. Moreover, samples such as environmental and

biological samples are complex in which the concentration of the target analytes is very low. Sample preparation methods

have aroused increasing interest in research on analytical the bioanalytical techniques field, as they have the key roles in

obtaining high clean-up and enrichment efficiency in the analysis of trace targets presented in the complex matrices. Recently,

except the traditional sample preparation methods (i.e. SPE and LLE), more and more new sample preparation methods are

applied prior to chromatography, spectrometry and electrochemistry analysis, for example, Dispersive Liquid-Liquid Micro

Extraction (DLLME), Salting-out Assisted Liquid-Liquid Extraction (SALLE), cloud point extraction (CPE), etc. In DLLME,

an appropriate mixture of extraction and disperser solvents is rapidly injected into an aqueous sample by a syringe. The fine

particle of extracting solvent that is dispersed into aqueous phase forms a cloudy solution and allows its interaction with the

analytes. The analytes are extracted from the sample to the fine droplets of extraction solvents. After centrifugation, phase

separation is achieved and the enriched analytes is analyzed by some instrumental methods. SALLE is based on the formation

of a biphasic system of mutual miscibility of two liquids by addition of additives, i.e. salts and sucrose. Considering the organic

solvents as extraction and protein precipitation agents, SALLE is an excellent alternative used for biological sample. CPE is

based primarily on the hydrophobic interaction between solutes and surfactants. It has become an alternative to conventional

solvent extraction due to several possible advantages such as low cost, environmental safety, high capacity to concentrate a

wide variety of analytes of widely varying nature with high recoveries and high enrichment folds.

Biography

Yingying Wen has been graduated with a BA degree and PhD from Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences. Further, she had

joined Hainan Medical University. Her research work focuses on sample preparation and chromatographic separation, including solid phase extraction and liquid

phase extraction techniques, as well as chromatographic separation methods such as LC/MS and GC/MS. She is the author and co-author of more than 20 original

research papers and several reviews.

wyy_418@163.com